Bl 5138

The generation of the ^UASBirA and ^UAS(^bioUb)₆-BirA flies and their recombination with elav^GAL4 for the studying of ubiquitinated material in the embryo nervous system development had been previously described [21 (link)]. For the analysis of the ubiquitinated material in Drosophila adult brain ^UASBirA and the ^UAS(^bioUb)₆-BirA flies were independently recombined with the eye specific Glass Multimer Reporter-GAL4 driver (GMR^GAL4). Flies carrying the GMR^GAL4 (BL 1104), the pan-neuronal elav-GAL4 (elav^GAL4; BL 8765) or the heat shock-GAL4 (Hs^GAL4; BL 2077) drivers on the second chromosome and flies carrying the tubulin-GAL4 (Tub^GAL4; BL 5138) and GAL80 (Tub^GAL80ts; BL 7017) drivers on the third chromosome were provided by the Bloomington Stock Center (Bloomington, IN, USA). Flies with the glutamatergic neuron-specific GAL4 driver (OK371^GAL4) on the second chromosome were kindly provided by Cahir O’Kane. Ube3a gain of function and loss of function mutants were a gift from Janice Fischer [34 (link)]. Flies overexpressing the C-terminal half of Rpn10 (^UASRpn10-ΔNTH) were a gift from Zoltan Lipinszki [35 (link)].

Flies were grown and maintained on regular corn medium, at 25 °C, in a 12 h/12 h light/dark cycle. Control Drosophila melanogaster used in this study were Oregon-R, P*82 (precise excision of P{SUPor-P}JhI-21^KG00977; electrophysiology and iGluR quantification), and w¹¹¹⁸ (electrophysiology, iGluR quantification , and behavior). JhI-21 mutants P{SUPor-P}JhI-21^KG00977 (BL12970), PJhI-21^EP1187 (not available any longer at Bloomington, but still available in our laboratory), and Df(2L)esc-P3-0 (BL3131) were previously characterized29 (link) and obtained from the Bloomington Stock Center. JhI-21 alleles were re-balanced over CyO-GFP. P{TubP- Gal4}LL7/TM3, Sb was obtained from Bloomington (BL5138) and re-balanced over TM3GFP, Ser. UAS-nSyb-GFP56 (link) provided by B. Hovemann, (Ruhr-University Bochum), repo-Gal4 (BL7415, obtained from Bloominton), and 24-B-Gal4 (BL1767, obtained from Bloomington), OK6-Gal4 and OK-371-Gal4 were provided by H. Abele (Heinrich-Heine University Düsseldorf). All genotypes used for behavioral experiments were backcrossed 5 times to an isogenized w¹¹¹⁸ fly stock.