Recombinant human IL-1β, IL-6, IL-8, RANTES, and MMP-1 ELISA assays were purchased from R&D Systems (Minneapolis, MN). PGG was purchased from Sigma (G7548 >95% pure) for in vitro and from Cayman Chemicals (#16007) for animal study. Thiamet G (#13237, Cayman Chemicals), human cytokine antibody array C5 (AAH-CYT-5-2) and TRANCE ELISA (ELM-TRANCE-1) from RayBiotech (Norcross, GA), rat Cytokine/Chemokine Magnetic Bead Panel (RECYMAG65K27PMX; Sigma, MO), mouse O-GlcNAc (CTD110.6) (sc-59623), mouse A20 (#sc-166692), and mouse monoclonal β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotech (Santa Cruz, CA). Antibodies against p-TAK1 Thr184/187 (#4531), p-JNK (#9251) p-JNK (#4511), p-c-Jun (Ser73) (#9164), MyD88 (D80F5) (#4283), CYLD (D6O5O) (#12797) were purchased from Cell Signaling Technology (Beverly, MA). Anti-TAK1 (ab109526) and anti-TRAF6 (ab33915) antibodies were from Abcam (Cambridge, MA). Goat anti-rabbit and goat anti-mouse HRP-linked secondary antibodies were purchased from Cell Signaling Technology.
Mmp 1 elisa
Manufactured by R&D Systems
Sourced in United States
The MMP-1 ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of Matrix Metalloproteinase-1 (MMP-1) levels in cell culture supernates, serum, and plasma.
Automatically generated - may contain errors
Lab products found in correlation
Myd88 d80f5, by Cell Signaling Technology (1 mentions)
Thiamet g, by Cayman Chemical (1 mentions)
Mouse monoclonal β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
P c jun ser73, by Cell Signaling Technology (1 mentions)
Rantes, by R&D Systems (1 mentions)
Timp1 elisa kits, by R&D Systems (1 mentions)
2 protocols using mmp 1 elisa
1
Cytokine and Signaling Pathway Analysis Protocol
2
Quantifying Matrix Metalloproteinases
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Levels of MMP1, MMP9, and TIMP1 in the supernatant of cell samples from each group were measured using MMP1 ELISA (R&D Systems, Minneapolis, MN, USA), MMP9 ELISA (ZY-MMP9-Mu, ImmunoClone, USA), and TIMP1 ELISA kits (R&D Systems, Minneapolis, MN, USA), respectively, according to the manufacturer’s instructions. The optical density (OD) value of each well was measured at an excitation wavelength of 450 nm using a microplate reader (MK3, Thermo, Pittsburgh, PA, USA), and the sample concentration was calculated according to the relationship between sample concentration and OD value.
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