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5 protocols using hydrochloric acid (hcl)

1

Pequi Endocarp Bioactivation and Characterization

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For this work, the following reagents were used: methylene blue (C16H18SN3Cl, Dinâmica, Brasilpaís), Sodium Hydroxide (NaOH, Dinâmica, 98%, Brazil), Hydrochloric Acid (HCl, Dinâmica, 38%, Brazil), Sodium Chloride (NaCl, Dinâmica, 99%, Brazil), Distilled Water (Brazil). All reagents were analytical in degree, and no previous purification was required. The pequi endocarp residues were obtained at the free fair of the municipality of Floriano (PI), Avenida Bucar Neto, Centro, Floriano (PI), CEP 64800-000.
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2

Cylindrospermopsin Quantification Protocol

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Cylindrospermopsin standard (purity > 95%) was purchased from Eurofins/Abraxis (Eurofins/Abraxis, Warminster, PA, USA). Methanol (99.9% HPLC grade), Ferrozine (97%), and peroxidase from horseradish (type II) were obtained from Sigma-Aldrich (Sigma-Aldrich, São Paulo, SP, Brazil). Acetic acid glacial (99.7% HPLC grade) was purchased from J.T Baker (J. T. Baker, Brazil). Hydroxylamine hydrochloride (96%) and ammonium hydroxide (27% v/v) were purchased from Synth (Synth, Diadema, SP, Brazil). Ammonium acetate (97%), N,N-Diethyl-p-phenylenediamine sulfate salt (98%), sodium phosphate dibasic (98%), sodium phosphate monobasic (98%), and hydrogen peroxide (35% v/v) were obtained from Neon (Neon, Suzano, SP, Brazil). Sodium sulfite (98%), sulfuric acid (98% v/v), hydrochloric acid (36.5% v/v), iron (II) sulfate heptahydrate (99%) and iron (III) chloride hexahydrate (97%) were purchased from Dinâmica (Dinâmica, Indaiatuba, SP, Brazil).
All solutions used in the experiments were prepared using ultrapure water (Milli-Q Reference water purification system, C79625, Merck Millipore, Darmstadt, Hesse, Germany).
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3

Enzymatic Biodiesel Production from Soybean Oil

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Analytical grade reagents were used to prepare all solutions. Sulfuric acid (98 wt%) was acquired from Vetec®, hydrochloric acid (37 wt%) were purchased from Dinâmica®; isopropyl alcohol (99.5 wt%), sodium periodate (99.8 wt%), monoethylene glycol (99 wt%), sodium hydroxide (>97 wt%) and potassium hydroxide (>97 wt%) were purchased from Neon. Absolute methanol (99.95 wt%), absolute ethanol (99.95 wt%), THF and ammonium hydroxide (99.8 wt%) were acquired from Merck®. lauric acid (99.8 wt%), Candida antarctica Lipase A (CAL-A) recombinant from Aspergillus oryzae, and 4 Å molecular sieves were purchased from Sigma-Aldrich®. Lipozyme 435 and lipozyme TLIM were purchased from Novozyme®, and Soya® soybean oil was acquired at local market.
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4

Extraction and Characterization of Anthocyanins

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Raw Bent sample, donated by the Bentonisa company (Boa Vista, Paraíba, Brazil), was used as precursor material. Hexadecyltrimethylammonium bromide (CTAB) (Êxodo Científica, Sumaré, Brazil), ammonium acetate (Êxodo Científica, São Paulo, Brazil), anthocyanin (ACN) (elderberry extract—SCIYU BIOTECH CO., LIMITED, Xi’an, China), hydrochloric acid (Dinâmica, São Paulo, Brazil), sodium hydroxide (Êxodo Científica, São Paulo, Brazil), and ethanol (Dinâmica, São Paulo, Brazil) were analytical grade and used without prior purification. Distilled water was used in all preparations
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5

Whey Protein Isolate Microgel Fabrication

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Whey Protein Isolate (WPI) 88% purity (CL 3987, Alibra Ingredients, Campinas, Brazil) was used to prepare WPI microgels. Tannic acid (99%, Sigma-Aldrich, Darmstadt, Germany—1701.20 g mol−1) and citric acid (99%, Synth, Diadema, Brazil—192.12 g mol−1) were used as crosslinking agents. Sodium azide (99%, 0.1 g L−1, Dinâmica, Indaiatuba, Brazil) was used to prevent microbial growth. Sodium hydroxide and hydrochloric acid solutions (HCl, 0.1 mol L−1, Dinâmica, Indaiatuba, Brazil) were used for pH adjustment. Roasted coffee oil was kindly donated by Linax—Essential Oils (Votuporanga, Brazil).
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