Pollen size analysis using the Beckman Multisizer II Coulter counter was performed as previously described (De Storme et al., 2013 (link)). Briefly, a single tomato flower at anthesis was vortexed for a couple of seconds in 1ml of Isoton II. Afterwards the flower debris was removed and the suspension was diluted in another 10ml of Isoton II before automatic measurement on the Multisizer II. Data were analyzed using custom R scripts.
Multisizer 2 coulter counter
Manufactured by Beckman Coulter
The Multisizer II Coulter counter is a laboratory instrument designed for the measurement and analysis of particle size and concentration. It utilizes the Coulter principle to detect and count particles suspended in an electrolyte solution, providing objective and reliable data on particle characteristics.
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Lab products found in correlation
2 protocols using multisizer 2 coulter counter
1
Tomato Pollen Size Analysis
2
Cell Diameter Measurement via Coulter Counter
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A 256 channel Multisizer II Coulter Counter (Beckman Coulter, Fullerton, CA) was used to determine the mean diameter of the cell population. Cells were diluted in Isoton® (Beckman Coulter) to a final volume of 15 mL. The counting conditions were: 500 μL sample volume, cumulating three successive assays. Results were displayed as the mean ± standard deviation for three independent experiments.
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