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Nkp46 m20

Manufactured by Santa Cruz Biotechnology
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NKp46 (M20) is a product offered by Santa Cruz Biotechnology. It is a monoclonal antibody that specifically recognizes the NKp46 receptor, which is expressed on the surface of natural killer cells. The core function of this product is to enable the detection and analysis of NKp46-positive cells.

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Lab products found in correlation

Deoxyribonuclease 1, by Merck Group (3 mentions) Phosphate buffered saline tablet, by Merck Group (3 mentions) Penicillin g, by Thermo Fisher Scientific (3 mentions) Hoechst, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Hematoxylin, by Merck Group (2 mentions) Bovine serum albumine (bsa), by Merck Group (2 mentions) Secondary abs, by Agilent Technologies (2 mentions) Percoll, by Merck Group (2 mentions) Rabbit anti iba1, by Fujifilm (2 mentions) Glucose, by Merck Group (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Cxcl12, by Thermo Fisher Scientific (1 mentions) Recombinant human egf, by Thermo Fisher Scientific (1 mentions) Rt pcr system, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Immunological Sciences (1 mentions) Microbeads cd11b, by Miltenyi Biotec (1 mentions) Transwell insert, by BD (1 mentions) Lipopolysaccharides (lps), by Immunotools (1 mentions) Secondary ab, by Thermo Fisher Scientific (1 mentions)

3 protocols using nkp46 m20

1

Isolation and Analysis of Microglia

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All Culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, glutamine, Na pyruvate, recombinant human EGF, Thermo Script RT‐PCR System, anti‐Acetyl‐alpha Tubulin (6‐11B‐1), anti‐Acetyl‐alpha Tubulin lys40 Abs and Hoechst (#33342, RRID:AB_10626776) were from GIBCO Invitrogen (Carlsbad, CA). Glucose, hematoxylin, eosin, Percoll, Papain (#P3125), phosphate buffered‐saline (PBS) tablet (#P4417), Bovine Serum Albumine (BSA) and deoxyribonuclease I were from Sigma‐Aldrich (Milan, Italy). Transwell inserts were from BD Labware (Franklin Lakes, NJ). Ki67 (#12202, RRID:AB 2620142) Ab were from Cell Signaling (Danvers, MA). NKp46 (M20) (#sc‐18,161, RRID: AB_2149152) antibody (Ab) was from Santa Cruz biotechnology (Santa Cruz, CA). Anti‐alpha tubulin (ab52866) and anti‐TMEM119 Abs were from Abcam (Cambridge, UK). Secondary Abs were from DAKO (Milan, Italy). CXCL12 was from Peprotech. LPS is from Immunotools (Friesoythe, Germany). IFN‐γ were from Immunological Sciences (Rome, Italy). Microbeads CD11b+ were from Miltenyi Biotec (Bologna, Italy). CD45, CD69, CD107a, NK1.1 Abs were from eBioscience Inc. (San Diego, CA). Rabbit anti‐Iba1 from Wako (VA).
Mormino A., Cocozza G., Fontemaggi G., Valente S., Esposito V., Santoro A., Bernardini G., Santoni A., Fazi F., Mai A., Limatola C, & Garofalo S. (2021). Histone‐deacetylase 8 drives the immune response and the growth of glioma. Glia, 69(11), 2682-2698.
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2

Isolation and Characterization of Murine Immune Cells

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All cell culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, secondary Abs, and Hoechst (catalog #33342, RRID:AB_10626776) were from GIBCO Invitrogen (Carlsbad, CA, USA). Phosphate buffered-saline (PBS) tablet (#P4417), Bovine Serum Albumine (BSA) and deoxyribonuclease I, were from Sigma-Aldrich (Milan, Italy). NKp46 (M20) (#sc-18161, RRID:AB_2149152) antibody (Ab) was from Santa Cruz biotechnology (Santa Cruz, CA). RNeasy Mini Kit was from Qiagen (Hilden, Germany). Fluorochrome-conjugated anti-CD45 (clone 104), -CD3 (clone 145-2C11), -CD4 (clone RM4-5), -CD8 (clone 53-6.7), -CD44 (clone IM7), -CD62L (clone MEL-14), -NK1.1 (clone pk136), -NKp46 (clone 29A1.4), -CD19 (clone 6D5), -CD49a (clone Ha31/8), -CD49b (clone DX5), -CD11b (clone M1/70), Gr1 (RB6-8C5), Ly6C (clone HK1.4), and F4/80 (BM8) mAbs were from eBioscience Inc., Biolegend or BD biosciences (San Diego, CA).Rat anti–IFN-γ monoclonal antibody (clone: XMG1.2) (Cat# BE0055, RRID:AB 1107694), anti-NK1.1 (Cat# BE0036, RRID:AB_1107737), and anti-VLA4 (CD49d/Natalizumab) (#BE0071, RRID:AB_1107657) were from Bioxcell (West Lebanon, USA).
Garofalo S., Cocozza G., Mormino A., Bernardini G., Russo E., Ielpo D., Andolina D., Ventura R., Martinello K., Renzi M., Fucile S., Laffranchi M., Mortari E.P., Carsetti R., Sciumè G., Sozzani S., Santoni A., Tremblay M.E., Ransohoff R.M, & Limatola C. (2023). Natural killer cells and innate lymphoid cells 1 tune anxiety-like behavior and memory in mice via interferon-γ and acetylcholine. Nature Communications, 14, 3103.
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3

Isolation and Characterization of Mouse NK Cells

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All culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, glutamine, Na pyruvate, ThermoScript RT-PCR Systems, Hoechst (#33342, RRID : AB_10626776), and mouse anti-IL-15 (PA5-47014) were from Gibco Invitrogen (Carlsbad, CA, USA). Glucose, hematoxylin, eosin, Percoll, Papain (#P3125), phosphate buffered-saline (PBS) tablet (#P4417), bovine serum albumin (BSA), and deoxyribonuclease I were from Sigma-Aldrich (Milan, Italy). NKp46 (M20) (#sc-18161, RRID: AB_2149152) antibody (Ab) and Arg1 (E-2) (#sc-271430) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Secondary Abs were from DAKO (Milan, Italy). Rabbit anti-Iba1 was from Wako (VA, USA). Anti-mouse (clone name in brackets) CD45 (104), CD69 (H1.2F3), CD3 (145-2C11), NKG2D (CX5), and NK1.1 (Pk136) mAbs directly conjugated to FITC, PE, PerCP 5.5, allophycocyanin, and allophycocyanin-eFluor 780 were from Ebioscences (Thermo Fisher Scientific, Waltham, MA USA). The pAAV-CMV-IL-15-GFP vector (CW304678) expressing the mouse IL-15 ORF under the control of a Cytomegalovirus promoter was from Tema Ricerca (Bologna, Italy).
Mormino A., Bernardini G., Cocozza G., Corbi N., Passananti C., Santoni A., Limatola C, & Garofalo S. (2021). Enriched Environment Cues Suggest a New Strategy to Counteract Glioma: Engineered rAAV2-IL-15 Microglia Modulate the Tumor Microenvironment. Frontiers in Immunology, 12, 730128.
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