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Angiotensinogen 1 14 renin substrate porcine

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Angiotensinogen 1–14 renin substrate porcine is a laboratory reagent used in the study of the renin-angiotensin system. It serves as a substrate for the enzyme renin, which cleaves the substrate to produce angiotensin I, a key intermediate in the regulation of blood pressure and fluid balance.

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Lab products found in correlation

Thin hematocrit capillaries, by Thermo Fisher Scientific (2 mentions) Competitive radioimmunoassay for angiotensin 1, by Tecan (2 mentions) Aldosterone i125 radioimmunoassay, by Tecan (2 mentions)

Close spelling variants of this product

Porcine angiotensinogen Angiotensinogen Substrates

2 protocols using angiotensinogen 1 14 renin substrate porcine

1

Aldosterone and Renin Activity Assay

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Plasma was collected in a stress-free environment by rapid non-terminal retro-orbital blood draw using thin hematocrit capillaries (Fisher Scientific). Aldosterone levels were determined on heparinized plasma (pre-diluted 2.5 times in PBS + 0.1% BSA) using an aldosterone I125 radioimmunoassay according to the manufacturer’s instructions (Tecan). Plasma renin activity was determined on EDTA-collected plasma using a competitive radioimmunoassay for Angiotensin I following the manufacturer’s instructions (Tecan). To rule out the impact of limiting amounts of angiotensinogen substrate in the plasma, an excess (about 180 ng) of angiotensinogen 1–14 renin substrate porcine (Sigma) was added to every sample, calibrator and control before quantification.
Pignatti E., Leng S., Yuchi Y., Borges K.S., Guagliardo N.A., Shah M.S., Ruiz-Babot G., Kariyawasam D., Taketo M.M., Miao J., Barrett P.Q., Carlone D.L, & Breault D.T. (2020). Beta-Catenin Causes Adrenal Hyperplasia by Blocking Zonal Transdifferentiation. Cell reports, 31(3), 107524.
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2

Aldosterone and Renin Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma was collected in a stress-free environment by rapid non-terminal retro-orbital blood draw using thin hematocrit capillaries (Fisher Scientific). Aldosterone levels were determined on heparinized plasma (pre-diluted 2.5 times in PBS + 0.1% BSA) using an aldosterone I125 radioimmunoassay according to the manufacturer’s instructions (Tecan). Plasma renin activity was determined on EDTA-collected plasma using a competitive radioimmunoassay for Angiotensin I following the manufacturer’s instructions (Tecan). To rule out the impact of limiting amounts of angiotensinogen substrate in the plasma, an excess (about 180 ng) of angiotensinogen 1–14 renin substrate porcine (Sigma) was added to every sample, calibrator and control before quantification.
Pignatti E., Leng S., Yuchi Y., Borges K.S., Guagliardo N.A., Shah M.S., Ruiz-Babot G., Kariyawasam D., Taketo M.M., Miao J., Barrett P.Q., Carlone D.L, & Breault D.T. (2020). Beta-Catenin Causes Adrenal Hyperplasia by Blocking Zonal Transdifferentiation. Cell reports, 31(3), 107524.
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