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Gfp g1546 antibody

Manufactured by Merck Group

The GFP (G1546) antibody is a laboratory reagent used to detect and localize green fluorescent protein (GFP) in various biological samples. It is a highly specific antibody that recognizes the GFP protein, allowing researchers to visualize and study the expression, distribution, and dynamics of GFP-tagged proteins in cells and tissues.

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2 protocols using gfp g1546 antibody

1

Western Blot Protocol for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were counted, normalized for cell number, lysed in SDS sample buffer, separated by electrophoresis on NuPage 4–12% Bis-Tris gels (Novex) and blotted onto nitrocellulose membranes (GE Healthcare). Antibodies for PTBP1 (ab5642), Drosha (ab12286), DICR (ab14601), EXOSC6 (ab50910), EXOSC10 (ab50558), and PARN (ab188333) were obtained from Abcam. Antibodies for Upf1 (A300-036A), METTL3 (A301-567A), EXOSC4 (A303-775A), EXOSC5 (A303-887A), and Xrn1 (A300-443A) were obtained from Bethyl Labs. The antibody against ZAP (16820-1-AP) was obtained from Proteintech. The HIV-1 capsid antibody (183-H12-5C) was obtained from the NIH AIDS reagent repository. The GFP (G1546) antibody was obtained from Sigma. The HIV Env (12-6205-1) antibody was obtained from American Research Products. The HA (HA.11) antibody used in the CLIP assays was obtained from Biolegend.
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2

Western Blot Protocol for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were counted, normalized for cell number, lysed in SDS sample buffer, separated by electrophoresis on NuPage 4–12% Bis-Tris gels (Novex) and blotted onto nitrocellulose membranes (GE Healthcare). Antibodies for PTBP1 (ab5642), Drosha (ab12286), DICR (ab14601), EXOSC6 (ab50910), EXOSC10 (ab50558), and PARN (ab188333) were obtained from Abcam. Antibodies for Upf1 (A300-036A), METTL3 (A301-567A), EXOSC4 (A303-775A), EXOSC5 (A303-887A), and Xrn1 (A300-443A) were obtained from Bethyl Labs. The antibody against ZAP (16820-1-AP) was obtained from Proteintech. The HIV-1 capsid antibody (183-H12-5C) was obtained from the NIH AIDS reagent repository. The GFP (G1546) antibody was obtained from Sigma. The HIV Env (12-6205-1) antibody was obtained from American Research Products. The HA (HA.11) antibody used in the CLIP assays was obtained from Biolegend.
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