Fab14001p

Manufactured by R&D Systems

The FAB14001P is a laboratory equipment product manufactured by R&D Systems. It is designed to perform a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Rab11, by Cell Signaling Technology (1 mentions) Lysotracker, by Cell Signaling Technology (1 mentions) Axio observer microscope, by Zeiss (1 mentions) Cd4 fitc, by R&D Systems (1 mentions)

2 protocols using fab14001p

Immunofluorescence Analysis of IL23R

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Cells were fixed in 4% paraformaldehyde, permeabilised and incubated with allophycocyanin-labelled anti-IL23R (FAB14001P, R&D Systems), Rab11, Rab7 (Cell Signaling), or DAPI (Thermo Fisher) and Cy2-labelled secondary antibodies. For LysoTracker (Cell Signaling) cells were stained prior to fixation. Fluorescence microscopy used the Zeiss Axio Observer microscope (Carl Zeiss Microscopy, Thornwood, NY). Colocalisation was quantified using ImageJ.

Sun R., Hedl M, & Abraham C. (2019). IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes. Gut, 69(2), 264-273.

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IL-23R Expression in CD4+ Cells

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PBMCs were isolated from HCs (n = 8) and SLT patients (n = 7), and stained for CD4 (CD4-FITC) and the IL-23 receptor (IL23R-PE; R&D Systems, cat#FAB14001P) and analysed with FACS. The percentage of CD4⁺ and CD4⁻ cells expressing the IL23-R were determined and compared between groups.

Evans R.D., Antonelou M., Sathiananthamoorthy S., Rega M., Henderson S., Ceron-Gutierrez L., Barcenas-Morales G., Müller C.A., Doffinger R., Walsh S.B, & Salama A.D. (2020). Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nature Communications, 11, 4368.

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