Ab205300

Manufactured by Abcam

Ab205300 is a lab equipment product offered by Abcam. It is a specialized tool designed for use in scientific research and laboratory settings. The core function of this product is to provide a specific capability or feature that supports various experimental and analytical procedures. However, without more detailed information about the product's specifications and intended use, a concise and unbiased description cannot be provided while maintaining an objective and factual approach. As a result, a comprehensive description is not available at this time.

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Lab products found in correlation

2 protocols using ab205300

Immunohistochemical Analysis of Tissue Samples

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Tissues were fixed in formalin overnight, then transferred to 70% ethanol, and embedded in paraffin (ARUP histology core). Formalin-fixed paraffin-embedded sections (4 micron) were used for hematoxylin and eosin and IHC staining. Antigen retrieval was performed by boiling slides for 20 min in 0.01 M citrate buffer, pH 6.0. Slides were blocked for 15 min with 3% H₂O₂, followed by 5% goat serum in PBS containing 0.1% Tween-20 (PBST). Primary antibodies were incubated overnight at 4 °C and include the following: BCL2 (#M088701-2, clone 124, Agilent), MYC (ab32072, Abcam), NEUROD1 (ab205300, Abcam), and ASCL1 (#556604, BD Pharmingen). Slides were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Vector Laboratories, 1:200) and developed with DAB (Vector Laboratories). A Nikon Eclipse Ci microscope and DS-Fi3 camera were used for imaging.

Dammert M.A., Brägelmann J., Olsen R.R., Böhm S., Monhasery N., Whitney C.P., Chalishazar M.D., Tumbrink H.L., Guthrie M.R., Klein S., Ireland A.S., Ryan J., Schmitt A., Marx A., Ozretić L., Castiglione R., Lorenz C., Jachimowicz R.D., Wolf E., Thomas R.K., Poirier J.T., Büttner R., Sen T., Byers L.A., Reinhardt H.C., Letai A., Oliver T.G, & Sos M.L. (2019). MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer. Nature Communications, 10, 3485.

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Comprehensive Protein Expression Analysis in Stem Cell Research

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Antibodies against octamer-binding transcription factor 4 (1:1000, Oct-4; ab200834, abcam), Brachyury (1:1000, sc-166962, Santa Cruz Biotechnology), GATA-binding protein 4 (1:1000, GATA4; sc-25310, Santa Cruz Biotechnology), SRY box transcription factor 17 (1:1000, Sox17; AF1924, R&D system), neuronal differentiation 1 (1:1000, NeuroD1; ab205300, abcam), XRCC5 (1:2000, AF5819, R&D system), IGF2BP1 (1:1000, ab184305, abcam), RBBP4 (1:1000, MAB7416, R&D system), NPM1 (1:1000, sc-271737, Santa Cruz Biotechnology), PCNA (1:1000, sc-56, Santa Cruz Biotechnology), GNAI2 (1:1000, ab137050, abcam), PHB2 (1:1000, sc-133084, Santa Cruz Biotechnology), LAMB1 (1:1000, sc-17810, Santa Cruz Biotechnology), LAMC1 (1:1000, sc-17751, Santa Cruz Biotechnology), β-catenin (1:1000, sc-7963, Santa Cruz Biotechnology), AKT (1:2000, #4691, Cell Signaling Technology), Jun amino-terminal kinases (JNK) (1:1000, #9252, Cell Signaling Technology), phosphorylated (p)-JNK (1:1000, #9255, Cell Signaling Technology), p38 (1:1000, sc-81621, Santa Cruz Biotechnology), p-p38 (1:1000, #9216, Cell Signaling Technology), TGF-β (1:1000, #3711, Cell Signaling Technology), CTGF (1:1000, sc-385970, Santa Cruz Biotechnology), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; (1:3000, sc-47724, Santa Cruz Biotechnology) were employed for the western blot analysis.

Seo J.H, & Jeon Y.J. (2021). Global Proteomic Analysis of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells via Connective Tissue Growth Factor Treatment under Chemically Defined Feeder-Free Culture Conditions. Journal of Microbiology and Biotechnology, 32(1), 126-140.

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