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Pyrogent 5000 kit

Manufactured by Lonza
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Sourced in Canada

The PYROGENT-5000 kit is a laboratory equipment product manufactured by Lonza. It is designed to detect and quantify endotoxin levels in a variety of samples. The kit utilizes a kinetic chromogenic method to determine the presence and concentration of endotoxin.

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Lab products found in correlation

Spectramax m3 microplate reader, by Molecular Devices (2 mentions)

2 protocols using pyrogent 5000 kit

1

Serum LPS Concentration Measurement

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Serum LPS concentrations were measured using PYROGENT-5000 kit as per manufacturer’s instructions (Lonza, Mississauga, ON, Canada). The absorbance was measured at 340 nm per minute for 1 h at 37 °C using a SpectraMax® M3 Microplate Reader (Molecular Devices, LLC. Sunnyvale, CA, USA). Reaction time was defined as the time required for the absorbance to increase 0.03 absorbance units.
Singh V.P., Fontaine M.A., Mangat R., Fouhse J.M., Diane A., Willing B.P, & Proctor S.D. (2021). High Vaccenic Acid Content in Beef Fat Attenuates High Fat and High Carbohydrate Western Diet Induced Changes in Lipid Metabolism and Gut Microbiota in Pigs. Microorganisms, 9(12), 2517.
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2

Quantifying Plasma LPS Levels

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Plasma LPS concentrations were measured using PYROGENT‐5000 kit as per manufacturer's instructions (Lonza, Mississauga, Canada). Samples were diluted 1:80 and heated for 10 min at 70°C. The absorbance was measured at 340 nm min−1 for 1 h at 37°C using a SpectraMax® M3 Microplate Reader (Molecular Devices, LLC. Sunnyvale, CA, USA).
Fouhse J., Yang K., Li J., Mills E., Ju T., Alvarado C.S., Chan C.B, & Willing B.P. (2018). Establishing a model for childhood obesity in adolescent pigs. Obesity Science & Practice, 4(4), 396-406.
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