8400s fourier transform infrared spectrophotometer

The FTIR analysis of femur cortical bones was done by using 8400S-Fourier Transform Infrared Spectrophotometer (SHIMADZU), with a resolution 4 cm⁻¹; scan speed 2.5 kHz, and 128 scans co-addition, in the KBr pellet form. Savitzky-Golay algorithm was used to eliminate the noise for obtaining smooth spectra of samples. The samples were held in contact with a prism made of highly refractive material transmitting infrared rays which are made incident on the sample at angle that induces total reflection. Bones were kept in 1% Triton, washed after 24 hours and dried under 60 W lamps for 6 hours followed by high vacuum drying. Dry bones were made into powder form and mixed with KBr (potassium bromide) in 1:100 ratios and analysed for FTIR.

The FTIR analysis of femur cortical bones was done by using 8400S-Fourier Transform Infrared Spectrophotometer (SHIMADZU), working on resolution 4 cm⁻¹; scan speed 2.5 kHz, and 128 scans co-addition, in the KBr pellet form. With the aid of Savitzky-Golay algorithm noise was eliminated to obtain smooth spectra of samples. The samples were held in contact with a prism made of highly refractive material transmitting infrared rays which are made incident on the sample at angle that induces total reflection. Bones were kept in 1% Triton, washed after 24 h and dried under 60 W lamps for 6 h followed by high vacuum drying. Dry bones were made into powder form and mixed with KBr (potassium bromide) in 1:100 ratios and analyzed for FTIR.

Particle size of the PR-GO nanosheets was measured by laser diffractometry using a nano size particle analyzer (Zen 1600 Malvern USA) in the range between 0.6 nm and 6.0 μm. Transmission Electron Microscopy was performed on a Tecnai G² spirit Biotwin (FP 5018/40), operating at an accelerating voltage of 80 kV. XRD measurements of PR-GO was taken with a PW 3040/60 PANalytical X-ray diffractometer that operated at a voltage of 45 KV and current of 30 mA with Cu Kα radiation (λ 1.54443 Ǻ). The diffracted intensities were recorded from 35° to 90° 2θ angles. EDX analysis was carried out with a Hitachi S 3400N, Japan, to identify the elemental compositions of the particles. The polysaccharide (PS), GO and PR-GO, were all analysed by Fourier transform infrared (FTIR) spectroscopy (Shimadzu 8400S fourier transform infrared spectrophotometer). The system worked in a diffuse reflectance mode at a resolution of 4 cm^-1 in KBr pellets. The scanning data were obtained from the average of 50 scans in the range of 4000 to 400 cm^-1. Raman spectra was monitored using 1.96 eV (633 nm) line of a He Ne laser in HORIBA-JOBIN-YVON Lab RAM HR 800 instrument by placing the sample solution into a semi-micro stopper cuvette with an exposure time of 1 s.