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Mrna wg 6 v3 microarray platform

Manufactured by Illumina

The Illumina MRNA WG-6 v3 microarray platform is a high-throughput gene expression profiling tool. It enables the measurement of the expression levels of thousands of genes simultaneously across multiple samples. The platform utilizes bead-based technology to generate robust and reproducible gene expression data.

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3 protocols using mrna wg 6 v3 microarray platform

1

Total RNA Extraction and mRNA Profiling

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Total RNA was isolated using the mirVana miRNA isolation kit (Life Technologies). mRNA expression profiling was performed using the Illumina mRNA WG-6 v3 microarray platform.
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2

miR-506-3p Overexpression Transcriptome Analysis

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mRNA expression array was conducted to examine the effect of miR-506-3p overexpression on gene expression as previously reported [20 (link)]. Total RNA was isolated using the miRVana miRNA isolation kit (Life Technologies, Carlsbad, CA, USA) as previously reported [30 (link)]. mRNA expression profiling was performed using the Illumina mRNA WG-6 v3 microarray platform as previously reported [20 (link)].
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3

Comprehensive RNA Expression Profiling

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Total RNA was isolated as previously described [46 (link)]. mRNA expression profiling was done using the Illumina mRNA WG-6 v3 microarray platform. miRNA expression was measured by qRT-PCR using TaqMan microRNA Assays (Life Technologies) with average expression of RNU44 RNA, RNU19 RNA, GAPDH mRNA and 18s rRNA used as controls for normalizing RNA loading.
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