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T hdca

Manufactured by Steraloids
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T-HDCA is a laboratory product used for chemical analysis. It serves as a reagent for various analytical procedures. The core function of T-HDCA is to facilitate chemical reactions and measurements as part of scientific research and testing activities.

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Lab products found in correlation

T udca, by Steraloids (5 mentions) Tcdca, by Merck Group (4 mentions) T β mca, by Steraloids (4 mentions) G lca, by Steraloids (3 mentions) G udca, by Steraloids (3 mentions) G dca, by Steraloids (3 mentions) T lca, by Steraloids (3 mentions) Gcdca, by Merck Group (2 mentions) Tβmca, by Merck Group (2 mentions) Tαmca, by Steraloids (2 mentions) T dca, by Steraloids (2 mentions) Dehydrocholic acid, by Merck Group (1 mentions) Lithocholic acid lca, by Merck Group (1 mentions) Anti cyp7a1, by Santa Cruz Biotechnology (1 mentions) Anti pparα antibody, by Abcam (1 mentions) Anti fxr, by Santa Cruz Biotechnology (1 mentions) Propranolol, by Merck Group (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Milli q ultrapure water purification system, by Merck Group (1 mentions) Minibest bacterial genomic dna extraction kit, by Takara Bio (1 mentions)

5 protocols using t hdca

1

Inflammatory Bowel Disease Biomarker Analysis

Cited 1 time
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OEA was purchased from Santa Cruz Biotechnology (Santa Cruz, CA); DSS (molecular weight=36,000–50,000 kDa) was obtained from MP Biochemical (Santa Ana, CA). MPA O-glucuronide and naloxone 3-β-D-glucuronide were obtained from Toronto Research Chemicals Inc. (ON, Canada). β-MCA, UDCA, HDCA, G-LCA, G-UDCA, G-DCA, T-β-MCA, T-UDCA, T-HDCA and T-LCA were purchased from Steraloids Inc. (Newport, RI). α-MCA, CA, DCA, CDCA, lithocholic acid (LCA), G-CDCA, G-CA, T-CDCA, T-DCA, T-CA, dehydrocholic acid and propranolol were purchased from Sigma-Aldrich (St Louis, MO). The anti-UGT1A, anti-FXR, anti-FGF15 and anti-CYP7A1 antibodies were purchased from Santa Cruz Biotechnology. The anti-PPARα antibody was from Abcam (Cambridge, UK). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from SunShine Biotechnology (Nanjing, China). The secondary antibodies and recombinant human FGF19 was obtained from Bioworld Technology (St Louis Park, MN). Other reagents, unless mentioned, were obtained from Sigma-Aldrich. RNA extracts of colon biopsies from 8 healthy humans and 13 ulcerative colitis and Crohn’s disease patients were previously described51 (link). The ulcerative colitis and Crohn’s disease samples were collected from consented patients and approved by the University of Michigan IRB committee (approval number HUM00042210).
Zhou X., Cao L., Jiang C., Xie Y., Cheng X., Krausz K.W., Qi Y., Sun L., Shah Y.M., Gonzalez F.J., Wang G, & Hao H. (2014). PPARα-UGT axis activation represses intestinal FXR-FGF15 feedback signalling and exacerbates experimental colitis. Nature Communications, 5, 4573.
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2

Comprehensive Bile Acid Profiling Protocol

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HPLC-grade methanol and acetonitrile were purchased from Fisher Scientific (Nepean, Ont., Canada). Ultrapure water was prepared by the Milli-Q Ultrapure water purification system (Millipore, Bedford, MA, USA). AOM was obtained from Sigma-Aldrich (St. Louis, MO, USA), and DSS (MW 36000-50000) was purchased from MP Biomedicals (Santa Ana, CA). Ammonium acetate, formic acid, glacial acetic acid and the other reagents (analytical grade) were purchased from Nanjing Chemical Factory (Nanjing, China). ‘Mouse Total Bile Acids Kit’ was purchased from Crystal Chem Inc (Downers Grove, United States). ‘MiniBEST Bacterial Genomic DNA Extraction Kit’ was from TaKaRa Bio Inc (Dalian, China). Anti-FXR, FGF15 and CYP7A1 antibodies were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti-GAPDH antibody was from Bioworld Technology (St. Louis Park, MN, USA).
CA, CDCA, DCA, and LCA, as well as their glycine [38 (link)] and taurine [45 (link)] conjugates, G-CA, G-CDCA, T-β-MCA, T-CA, T-CDCA, T-DCA, and internal standard dehydrocholic acid (dhCA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). α-MCA, UDCA, HDCA, G-LCA, G-UDCA, G-DCA, T-β-MCA, T-UDCA, T-HDCA and T-LCA were purchased from Steraloids, Inc. (Newport, Rhode Island, USA).
Cao L., Che Y., Meng T., Deng S., Zhang J., Zhao M., Xu W., Wang D., Pu Z., Wang G, & Hao H. (2017). Repression of intestinal transporters and FXR-FGF15 signaling explains bile acids dysregulation in experimental colitis-associated colon cancer. Oncotarget, 8(38), 63665-63679.
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3

Quantitative Bile Acid Profiling

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Cholic acid (CA), chenodeoxy Cholic acid (CDCA), deoxyCholic acid (DCA), lithoCholic acid (LCA), α muriCholic acid (αMCA), βMCA, ωMCA, ursodeoxyCholic acid (UDCA), hyodeoxyCholic acid (HDCA), murideoxyCholic acid (MDCA), taurine conjugated Cholic acid (T-CA), T-CDCA, T-DCA, T-LCA, T- αMCA, T- βMCA, T- ωMCA, T-UDCA, and T-HDCA were purchased from Steraloids (Newport, Rhode Island). T- ωMCA was given by Dr. Daniel Raftery’s laboratory at the University of Washington Northwest Metabolomics Research Center. For internal standards (ISs) , a total of 5 deuterated (d4) ISs were used. Among them the d4-Cholic acid (d4-CA) was purchased from Toronto Research Chemicals Inc (Ontario, Canada), the d4-deoxyCholic acid (d4-DCA) and d4-chenodeoxyCholic acid (d4-CDCA) were purchased from CDN Isotope Inc (Pointe-Claire, Quebec, Canada), the d4-glycine conjugated CDCA (d4-G-CDCA) was purchased from IsoSciences (Ambler, PA), and d4-lithoCholic acid (d4-LCA) was purchased from Steraloids (Newport, Rhode Island). One mg/mL stock solutions of the individual BAs (for standard curve) and IS were prepared in methanol and water (1:1). The 19 individual BA stock solutions were further diluted in 50% methanol to obtain 10 working standard solutions ranging from 0.05 to 10000 ng/mL). The 5 ISs were mixed to obtain a working IS solution in which the concentration of each IS was 10 µmol/mL.
Kim S., Choi S., Dutta M., Asubonteng J.O., Polunas M., Goedken M., Gonzalez F.J., Cui J.Y, & Gyamfi M.A. (2021). Pregnane X receptor exacerbates nonalcoholic fatty liver disease accompanied by obesity- and inflammation-prone gut microbiome signature. Biochemical pharmacology, 193, 114698.
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4

Bile Acid Panel Profiling Protocol

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β-MCA, ω-MCA, UDCA, HDCA, T-α-MCA, T-β-MCA, T-UDCA, and THDCA were purchased from Steraloids Inc. (Newport, RI). α-MCA, CA, DCA, CDCA, TCDCA, TDCA, and TCA were purchased from Sigma-Aldrich (St Louis, MO). Irinotecan was obtained from Sigma-Aldrich (St Louis, MO). All other reagents and solvents were of HPLC grade. Anti-mouse CD3 (clone 145-2C11), anti-mouse CD28 (clone 37.51), anti-mouse CD16/CD32 (clone 93), anti-mouse CD45 Alexa Fluor® 700 (Clone: 30-F11), anti-mouse CD4 eFluor® 450 (Clone: GK1.5), anti-mouse CD8α PE-Cyanine7 (Clone: 53-6.7), anti-mouse CD8β FITC (Clone: eBioH35-17.2), anti-mouse TCRβ PerCP-Cyanine5.5 (Clone: H57-597), anti-mouse/rat Foxp3 APC (Clone: FJK-16s), anti-mouse IL-10 APC (Clone: JES5-16E3) were from eBiosciences (San Diego, CA). Cytofix/Cytoperm Fixation/ Permeabilization Solution and Golgi Plug protein transport inhibitor were purchased from BD Biosciences (Franklin Lakes, NJ). Foxp3 Fix/Perm Buffer Set and mouse IL-10 ELISA kit were purchased from BioLegend (San Diego, CA).
Fang Z.Z., Zhang D., Cao Y.F., Xie C., Lu D., Sun D.X., Tanaka N., Jiang C., Chen Q., Chen Y., Wang H, & Gonzalez F.J. (2015). Irinotecan (CPT-11)-induced elevation of bile acids potentiates suppression of IL-10 expression. Toxicology and applied pharmacology, 291, 21-27.
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5

Comprehensive Bile Acid Quantification

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Deuterated-cholic acid (d4-CA), bile acids (CA, CDCA, LCA, TCA, UDCA, taurine, βMCA, TαMCA, TβMCA, TLCA, TCDCA), and formic acid were purchased from Sigma-Aldrich Chemicals Co. (St Louis, MO, USA). All other bile acid standards (GCDCA, GCA, GDCA, GHDCA, GLCA, GUDCA, HCA, HDCA, ILA, TDCA, THDCA, TUDCA) were purchased from Steraloids Inc. (Newport, RI, USA). Acetonitrile (ACN) and methanol (MeOH) of Optima LC-MS grade quality were purchased from Thermo Fisher Scientific (Auckland, New Zealand).
James S.C., Fraser K., Young W., Heenan P.E., Gearry R.B., Keenan J.I., Talley N.J., Joyce S.A., McNabb W.C, & Roy N.C. (2021). Concentrations of Fecal Bile Acids in Participants with Functional Gut Disorders and Healthy Controls. Metabolites, 11(9), 612.
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