2 carboxyethyl acrylate cea

Doxorubicin hydrochloride (DOX, Cayman Chemical), (N,N-dimethylacrylamide (DMAM, Sigma Aldrich, Burlington, MA, USA), 2-carboxyethyl acrylate (CEA, Sigma Aldrich), N,N′-bis(acryloyl)cystamine (CBA, Alfa Aesar), N,N′-methylenebisacrylamide (MBA, Acros Organics, Geel, Belgium), lithium phenyl (2,4,6-trimethylbenzoyl)phosphinate (LAP, Carbosynth), Span 80 (Sigma Aldrich), cyclohexane (Chempur), acetone (Chempur), dimethyl sulfoxide (DMSO, Fisher Bioreagents, Pittsburgh, PA, USA), and dialysis membrane (Spectrum™ Spectra/Por™ 2 RC Dialysis Membrane, MWCO: 12–14 kDa).

2-(Dodecylthiocarbonothioylthio)propionic acid (CTA, Sigma Aldrich, St. Louis, Missouri, USA) was used without further purification. Azobis(isobutyronitril) (AIBN, ≥99%, Fluka, St. Louis, MI, USA) was recrystallized from methanol and stored at −18 °C. Methyl acrylate (MA, Sigma Aldrich, St. Louis, MI, USA) and 2-carboxyethyl acrylate (CEA, Sigma Aldrich, St. Louis, MI, USA) were purified by passing through a column of basic alumina (Sigma Aldrich, St. Louis, MI, USA) to remove the inhibitor. Sodium MMT was purchased from Alfa Aesar, Kandel, Germany. Propylen glycol monomethyl ether acetate (PGMEA, p.a., J&K Scientific, Beijing, China) was used as received. The used three-arm star shaped RAFT agent S3 was synthesized following a literature known procedure [16 (link)].