Zein protein was provided by Sigma Aldrich (St. Louis, MO, USA). Sunflower oil containing 40 wt.% of oleic acid was obtained from Coreysa company (Sevilla, Spain). All emulsions were prepared using deionized water.
Zein protein
Manufactured by Merck Group
Sourced in United States
Zein protein is a type of plant-based protein derived from corn. It is a natural, biodegradable material with unique properties that make it useful for various applications in the laboratory setting. Zein protein serves as a core component in the production of certain lab equipment and devices. Its specific functions and intended uses are not provided in this factual, unbiased description.
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Lab products found in correlation
7 protocols using zein protein
1
Zein Protein Sunflower Oil Emulsion
2
Synthesis of Gold and Silver Nanoparticles
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Zein protein (CAS # 9010-66-6), hydrogen
tetrachloroaurate(III) trihydrate (HAuCl4), silver nitrate
(AgNO3), and SDS, all AR-grade obtained from Sigma–Aldrich,
were used as such without further purification. The double-distilled
water was used for all sample preparations.
tetrachloroaurate(III) trihydrate (HAuCl4), silver nitrate
(AgNO3), and SDS, all AR-grade obtained from Sigma–Aldrich,
were used as such without further purification. The double-distilled
water was used for all sample preparations.
3
Biopolymer-based Material Synthesis
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GO was purchased from Tsuno Rice Fine Chemicals Co., Ltd. (Wakayama, Japan) with purity ≥99%. Zein protein was provided by Sigma‐Aldrich Co., Ltd. (USA). All other chemicals and solvents were analytical grade, while Tween 80 and lecithin were food‐grade, supplied from Union Chemical 1986 Co., Ltd. (Thailand).
4
Okara Protein Extraction and Analysis
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Fresh okara was obtained from Mr. Bean – a soy product company, Singapore. Zein protein, BC and FS were purchased from Sigma Aldrich, Singapore. High performance liquid chromatography (HPLC)-grade deionized (DI) water, chloroform, isopropyl alcohol, ethyl acetate and acetonitrile were used as solvents for extraction and analysis. The release media was prepared using pancreatin and pepsin enzymes, and Tween 20 purchased from Sigma Aldrich, Singapore.
5
Natural Food Preservative Formulation
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Zein protein and rosemary essential oil, used as a natural food preservative, were provided by Sigma Aldrich (San Luis, MO, USA). Sunflower oil containing 40 wt.% of oleic acid was bought in the local supermarket. The gum solutions were prepared using KELTROL® diutan gum, donated by CP Kelco (Atlanta, GA, USA), with deionized water.
6
Characterization of Corn and Quinoa Flours
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Corn flour (moisture 6.98 g/100 g, protein 3.16 g/100 g, ash 1.04 g/100 g) was obtained from Herboeste (Buenos Aires, Argentina), quinoa flour (moisture 12.39 g/100 g, protein 14.52 g/100 g, ash 3.18 g/100 g) was purchased from Sturla (Buenos Aires, Argentina), corn starch from Droguería Saporiti (Buenos Aires, Argentina), and dry egg (moisture 20.3 g/100 g, protein 47.50 g/100 g, ash 3.26 g/100 g) and dry egg-white (moisture 3.32 g/100 g, protein 73.32 g/100 g, ash 4.54 g/100 g) from Ovobrand SA (Brandsen, Argentina). Xanthan (XG), locust bean gum (LBG), and zein protein were food-grade obtained from Sigma Chemical Co. (St. Louis, MO). Analytical grade NaCl, sunflower oil (AGD, Buenos Aires, Argentina), and distilled water were also used.
Moisture content of flour (corn and quinoa), starch, and proteins (dry egg, dry egg-white, and zein) was measured following the standardized protocol AACC 44-40 (AACC, 2000) . Total nitrogen content was determined according to 920.87 AOAC method; conversion factors 5.83 for quinoa flour and 6.25 for the rest of proteins was used (Table 1).
Moisture content of flour (corn and quinoa), starch, and proteins (dry egg, dry egg-white, and zein) was measured following the standardized protocol AACC 44-40 (AACC, 2000) . Total nitrogen content was determined according to 920.87 AOAC method; conversion factors 5.83 for quinoa flour and 6.25 for the rest of proteins was used (Table 1).
7
Microparticle Synthesis with Vitamin D3
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Vitamin D 3 cholecalciferol (≥ 99.8% purity), rutin hydrate (≥ 94% purity), zein protein and chitosan low molecular weight and other chemicals were purchased from Sigma (Sigma-Aldrich, Merck Sigma, Italy). Ultrapure water was used for analysis whereas distilled water was used for the preparation of microparticles. All the chemicals were of analytical grade.
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