7890b 5977a gc msd gc ms

For detailed methods of soil sample processing and secondary metabolite detection, we refer to our previously published paper [3 (link)]. In order to increase the accuracy of the entire analysis, we also included one quality control (QC) sample for every 10 samples. The QC sample was prepared by mixing the extracts of all samples in the same amount, and each QC sample was the same amount as the tested sample. Analysis of GC-MS metabolomics of soil samples was conducted on Agilent Technologies Inc. 7890B-5977A GC/MSD GC-MS (Santa Clara, CA, USA), which was carried out, as described in our recent publication [3 (link)]. Furthermore, the metabolite information was obtained by analyzing the KEGG database after comparing the data obtained in this study with the standard spectrum library.

The soil sample for GC-MS metabolomics analysis was prepared as described in our recent publication [3 (link)]. The reliability of the entire analysis was justified by inserting one quality control (QC) sample among every 10 samples. The QC sample was prepared by mixing the extract of all samples in equal volume, and the volume of each QC sample was the same as that of the tested sample. Furthermore, all soil samples were stored at room temperature for GC-MS metabolomics analysis, which was carried out and analyzed as described in our recent publication on a 7890B-5977A GC/MSD GC-MS (Agilent Technologies Inc., Santa Clara, CA, USA) [3 (link)]. The obtained data in this study were analyzed by comparing them with that of the standard spectrum library of the National Institute of Standards and Technology, while the obtained metabolite information in this study was searched against the KEGG database.

The methods of soil sample processing and secondary metabolite detection refer to the author’s previously published paper (Ren et al., 2022b (link)). In detail, one quality control (QC) sample was added to every 10 samples to demonstrate that the whole analysis was accurate. The QC sample was made by mixing the extracts of all the samples in the same amount, and each QC sample was the same size as the tested sample. Also, the soil samples were kept at room temperature for GC–MS metabolomics analysis. This test was done on a 7890B-5977A GC/MSD GC–MS (Agilent Technologies Inc., Santa Clara, CA, United States) as our recent publication (Ren et al., 2022b (link)). In this study, the data were analyzed by comparing them to the National Institute of Standards and Technology’s standard spectrum library. The information about metabolites was looked up in the KEGG database.