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Toledo delta 320

Manufactured by Mettler Toledo

The Toledo Delta 320 is an analytical balance designed for laboratory use. It provides accurate and precise measurements of weight, with a maximum capacity of 320 grams and a readability of 0.1 milligrams. The balance features a high-quality, durable construction to ensure reliable performance in the laboratory environment.

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5 protocols using toledo delta 320

1

Soil Quality Assessment after Crop Harvest

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After the crop harvest, soil samples were taken from each unit and measured soil pH and EC using pH (Mettler Toledo Delta 320) and EC meter (DDS-307A) and EC meter according to the described method in our previous study (Bashir et al., 2020a (link), Bashir et al., 2020b , Bashir et al., 2020c ). Bioavailable Cd contents from each potted soil were estimated using the 1 M NH4NO3 extractable technique described in our previous study (Bashir et al., 2018 (link)). After extraction and filtration, supernatant was analyzed for Cd concentration through atomic absorption spectroscopy (AAS) (AA-240FS Varian, USA). Soil organic matter contents were calculated by using the wet oxidation method. Similarly, soil available nitrogen, phosphrous and potassium contents were estimated as the method proposed by (Lu et al., 2000).
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2

Comprehensive Soil Nutrient Analysis Protocol

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Soil pH (SpH) was determined at a 1:5 (w/v) soil/distilled water ratio using a pH meter (Mettler Toledo Delta 320 (Zhai et al., 2015 (link))). Soil organic carbon (SOC) was determined using the potassium dichromate oxidation method (Partey et al., 2014 (link)). Soil available N (AN) was measured using the alkaline hydrolysis diffusion method (Wang et al., 2018 (link)). Soil available P (AP) and total P (TP) were detected by Mo–Sb colorimetry using an EdgeLight UV754 spectrophotometer (Shanghai, China (Zhai et al., 2015 (link))). Soil available K (AK) and total K (TK) were analyzed by ICP–OES (PerkinElmer, Waltham, USA (Yu et al., 2016 (link))). Soil total N (TN) was determined by a Kjeldahl nitrogen analyzer (KDN-812, Shanghai, China) after digestion with concentrated sulphuric acid (Zhai et al., 2015 (link)). Soil C/N is the ratio of SOC to TN.
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3

pH Measurement of Inoculated Samples

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The pH of the samples was measured with a Mettler Toledo Delta 320 pH meter, at room temperature. Readings were taken before inoculation (negative control), immediately after inoculation (T = 0) and then at every sampling point.
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4

Seawater pH Modulation for Culture Experiments

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In situ seawater, filtered by 0.22 μm glass microfiber filters, was used for the culture experiment, and the pH of seawater was altered by adding 0.1 mol/L HCl or 0.1 mol/L NaOH (NaOH was freshly prepared prior to use) to ranges of 7.20–8.40 and 7.40–8.65, respectively. The pH measurements were conducted using a pH meter (Mettler Toledo Delta 320, with a precision of 0.01 unit). The pH sensor was three-point calibrated with standard buffer solutions of pH = 4.01, pH = 7.00, and pH = 10.01 at 25 °C before measurement.
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5

Determination of pH in Honey Samples

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The pH of samples was determined according to the method described in the International Honey Commission (14) . A digital pH meter (Mettler Toledo Delta 320, Shah Alam, Malaysia) was calibrated at room temperature using pH buffer 4 and 7. A 10 g sample was dissolved in 75 mL distilled water. The solution was well mixed before the reading was recorded.
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