The NEALS biorepository provided deidentified samples with a separate document containing clinical information and subject IDs. Before assays, thawed aliquots were cleared by 10-minute centrifugation at 18,000g at 4°C. Protein concentrations for serum and CSF were determined using bicinchoninic acid assay total protein assay (Thermo Scientific, 23,225) to assure that sample protein content fell within the normal range for adults (serum 6.4–8.3 g/dL; CSF15–45 mg/dL) and did not differ between groups (data not shown).
Bicinchoninic acid assay total protein assay
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Bicinchoninic acid (BCA) assay total protein assay is a colorimetric detection and quantitation method. It is used to measure the total protein concentration in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bicinchoninic acid assay total protein assay
1
Biorepository Sample Preparation and Protein Assay
2
Serum Preparation for Protein Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
De-identified blood samples were collected by venipuncture during routine consecutive clinic visits for up to four visits. Serum was separated from whole blood within an hour of collection by centrifugation (2500rpm at 23 C) and stored at À80 C. Prior to assays, thawed aliquots were cleared by 10-min centrifugation at 18,000Âg at 4 C. Protein concentrations for serum were determined using bicinchoninic acid assay total protein assay (Thermo Scientific, Waltham, MA, USA, 23225) to assure that the sample protein content fell within normal range for adults (serum 6.4-8.3 g/dl).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!