MG-63 or U2OS cells were seeded in 12-well plates at a density of 1×105 cells/well. After trans-fection, the cells were trypsinized and harvested for staining using an Annexin V-fluorescein isothiocyanate (FITC)/prop-idium iodide (PI) Detection kit (Sigma-Aldrich; Merck KGaA) according to the manufacturer's protocol. Cells were analysed by flow cytometry (Becton, Dickinson and company). The FITC+/PI− and FITC+/PI+ fractions were considered early and late apoptotic cells, respectively. Analysis of flow data was performed using FlowJo version X.10.0.7-1 (FlowJo LLC).
Annexin 5 fluorescein isothiocyanate fitc prop idium iodide pi detection kit
Manufactured by Merck Group
Sourced in United States
The Annexin V-fluorescein isothiocyanate (FITC)/prop-idium iodide (PI) Detection kit is a laboratory reagent used to detect and quantify apoptosis and necrosis in cells. The kit contains Annexin V-FITC and propidium iodide, which are fluorescent dyes that bind to specific cellular components during different stages of cell death.
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Lab products found in correlation
2 protocols using annexin 5 fluorescein isothiocyanate fitc prop idium iodide pi detection kit
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Apoptosis Induction and Analysis
2
Annexin V/PI Apoptosis Assay
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Transfected cells in 6-well plates were collected for washing in phosphate buffered saline (PBS). After double-staining with Annexin V fluorescein isothiocyanate (FITC)/propidium iodide (PI) detection kit (Sigma-Aldrich, St. Louis, MO, USA) for 15 min, apoptosis cells were assayed by flow cytometer (Beckman Coulter, Brea, CA, USA).
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