Sc 67324

Cells were fixed in 96% ethanol and immune-stained with rat anti-integrin β1 (1:100; in-house produced), and rabbit anti-H1° (1:100; sc-67324 Santa Cruz, CA, USA).
The secondary antibodies used were fluorescein isothiocyanate-conjugated anti-rat-(1:100; F1763), or rhodamine-conjugated anti-rabbit-(1:200; T6778) immunoglobulins (both from Sigma, MO, USA).
Nuclei were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (H1200, Vector Laboratories, Youngstown, OH, USA). Cells were observed in an Olympus BX-50 microscope (Olympus Italia S.r.l., Segrate, Italy) equipped with Vario Cam B/W camera (Nikon Instruments S.p.A., Calenzano, Italy).

Proteins (15 μg) were separated by electrophoresis on denaturing 12.5% polyacrylamide slab gels (SDS-PAGE) and transferred to PVDF membrane (IPVH00010, Immobilon P, Millipore, MA, USA), as previously described (19 (link)). Samples on the membrane were visualized by staining with Ponceau Red (Sigma-Aldrich) for 5 min. Membranes were immune-stained with rabbit polyclonal anti-H1° antibodies (1:500, sc-67324 Santa Cruz), mouse monoclonal anti-Hsc70 antibodies (1:1,000, sc-7298, Santa Cruz), rabbit polyclonal anti-SUMO1 (1:500, S373B, Santa Cruz). The secondary antibodies were AP-conjugated anti-mouse (1:7,500, S372B) and anti-rabbit (1:7,500, S373B) IgGs (Promega Corp., Madison, WI, USA).