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Jwh 015

Manufactured by Cayman Chemical
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JWH-015 is a synthetic cannabinoid compound. It acts as a selective agonist for the CB2 cannabinoid receptor. JWH-015 is used in scientific research, particularly in the study of the endocannabinoid system.

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Lab products found in correlation

Jwh 073, by Cayman Chemical (1 mentions) Jwh 081, by Cayman Chemical (1 mentions) Jwh 210, by Cayman Chemical (1 mentions) Dopamine hydrochloride, by Merck Group (1 mentions) Cp55 940, by Cayman Chemical (1 mentions) Jwh 133, by Cayman Chemical (1 mentions) Win55 212 2, by Bio-Techne (1 mentions) Jwh015, by Merck Group (1 mentions) Isofluorane, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Sr144528, by Cayman Chemical (1 mentions) Wi 38, by Thermo Fisher Scientific (1 mentions) Cell culture materials, by Thermo Fisher Scientific (1 mentions) Z devd fmk, by Santa Cruz Biotechnology (1 mentions) Jwh 200, by Cayman Chemical (1 mentions) L α phosphatidylcholine epc, by Avanti Polar Lipids (1 mentions)

6 protocols using jwh 015

1

Synthetic Cannabinoid Compound Analysis

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THC and six SCs (AM-694, JWH-015, JWH-073, JWH-081, JWH-210, and RCS-4) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Eight additional SCs, including JWH-018, CRA13 and its derivatives (CRA13-F and CRA13-OH), and CP47,497 and its homologs (CP47,497-C6, C8, and C9) were synthesized. The synthetic methods are briefly described, and the proton nuclear magnetic resonance spectroscopic data of the synthesized compounds are attached in the Supplementary Materials.
Lee W., Park S.J., Hwang J.Y., Hur K.H., Lee Y.S., Kim J., Zhao X., Park A., Min K.H., Jang C.G, & Park H.J. (2020). QSAR Model for Predicting the Cannabinoid Receptor 1 Binding Affinity and Dependence Potential of Synthetic Cannabinoids. Molecules, 25(24), 6057.
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2

Preparation and Storage of Neuroactive Compounds

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WIN55,212-2 and BAY59-3074 (BAY) were purchased from Tocris Bioscience (Bristol, United Kingdom). CP55,940, AEA, 2-AG, JWH-015, and JWH-133 were purchased from Cayman Chemical Company (Ann Arbour, MI, United States). (-)-trans-THC was purchased from THC Pharm GmbH (Frankfurt, Germany). Dopamine hydrochloride was purchased from Sigma–Aldrich (St. Louis, MO, United States). Arginine vasopressin (AVP) was a kind gift from Dr. Mark Oliver (The University of Auckland, Auckland, New Zealand).
Drug stocks were prepared in absolute ethanol (CP55,940, WIN55,212-2, AEA, 2-AG, THC, BAY, JWH-133) or DMSO (JWH-015) and were stored in aliquots at -80°C prior to use. Dopamine and AVP were made up in H2O immediately prior to use. Drug aliquots used for experiments involving serial dilutions were always single-use. Vehicle controls for serial dilutions were maintained constant within experiments.
Ibsen M.S., Finlay D.B., Patel M., Javitch J.A., Glass M, & Grimsey N.L. (2019). Cannabinoid CB1 and CB2 Receptor-Mediated Arrestin Translocation: Species, Subtype, and Agonist-Dependence. Frontiers in Pharmacology, 10, 350.
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3

Cannabinoid and Opioid Receptor Agonist Protocol

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JWH015, a cannabinoid 2 receptor (CB2) agonist (CB2 Ki = 13.8 nM, 30 to 80 fold selectivity versus CB1) was obtained from Cayman Chemical (Ann Arbor, MI). JWH015 was dissolved in a vehicle solution of 10% dimethyl sulfoxide, 10% Tween-80, and 80% saline (Sigma, St. Louis, MO). The mu-opioid agonist (MOR) morphine sulfate was purchased from the NIDA Drug Supply program (Rockville, MD) and was dissolved in saline. Formalin solution was obtained by diluting 1.5% of formaldehyde in saline. Ketamine:xylazine (80 mg/kg: 12 mg/kg; Phoenix Pharmaceutical, St. Joseph, MO) was used to anesthetize animals in order to insert microdialysis probes into the nucleus accumbens. The antibiotic gentamicin (Phoenix Pharmaceutical, St. Joseph, MO) was provided as a single subcutaneous dose (8mg/kg). Drugs were weighed out and dissolved in vehicle daily, prior to use. Cocaine hydrochloride was purchased from the NIDA Drug Supply program (Rockville, MD) and used as a positive control in microdialysis and HPLC studies. Finally, 5% and 2.5% isofluorane (Sigma, St. Louis, MO) mixed in 2.5 L/min of oxygen was delivered through a nose cone and used to induce and maintain anesthesia, respectively, for paw incision and SNI surgeries.
Grenald S.A., Young M.A., Wang Y., Ossipov M.H., Ibrahim M.M., Largent-Milnes T.M, & Vanderah T.W. (2016). Synergistic attenuation of chronic pain using mu opioid and cannabinoid receptor 2 agonists. Neuropharmacology, 116, 59-70.
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4

Investigating Pirfenidone and Cannabinoid Effects on Lung Fibroblasts

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The human embryonic lung fibroblast cell line WI38 was purchased from Shanghai Institutes for Biological Sciences. WI38 cells were maintained in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum (both Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 U/ml streptomycin at 37°C in a humidified atmosphere with 5% CO2. At 50% confluency, the culture medium was replaced with serum-free DMEM for 24 h prior to treatment with BALF extracted from BLM-treated mice. BALF was prepared as previously described (20 (link)). Briefly, after 7 days of treatment with BLM, mice were sacrificed, and a plastic cannula was inserted into the trachea. A cold sterile saline solution was gently injected to perform bronchoalveolar lavage (BAL). The BALF was centrifuged at 700 × g at 4°C for 5 min, and the supernatant was stored at −80°C until further use.
WI38 cells were incubated with an equal volume of DMEM and BALF containing 200 µg/ml pirfenidone, 20 µM JWH-015 (a CB2R-selective agonist) or 1 µM SR144528 (a CB2R antagonist) for 48 h at 37°C. JWH-015 and SR144528 were obtained from Cayman Chemical Co. The concentration of drugs was determined according to a previous study (21 (link)).
Liu J, & Shi G. (2019). Pirfenidone activates cannabinoid receptor 2 in a mouse model of bleomycin-induced pulmonary fibrosis. Experimental and Therapeutic Medicine, 18(6), 4241-4248.
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5

CB1 and CB2 Receptor Antagonists Effects

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Rimonabant (SR141716A) and SR144528 (National Institute on Drug Abuse, Bethesda, MD) were dissolved in a 1:1:18 (ethanol:cremaphor:saline) solution, which served as the vehicle. In cellular assays, these antagonists demonstrate binding selectivity for CB1 (rimonabant) or CB2 (SR144528) receptors, although they may act as inverse agonists at high concentrations (Govaerts et al., 2004 (link)). In male rats, rimonabant doses as low as 0.32-1 mg/kg i.p. antagonize THC's antinociceptive and motoric effects, whereas SR144528 doses as high as 10 mg/kg i.p. do not (Craft et al., 2012 (link)). JWH015 was purchased from Cayman Chemical (Ann Arbor, MI); it was dissolved in DMSO to which distilled water was added for a final DMSO concentration of 75%. All drugs were administered i.p. in volumes of 1-2 ml/kg.
Craft R.M., Greene N.Z, & Wakley A.A. (2018). Antinociceptive effects of JWH015 in female and male rats. Behavioural pharmacology, 29(2-), 280-289.
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6

Lipid-Based Nanoparticle Synthesis Protocol

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All chemicals and drugs were purchased from Sigma (St. Louis, MO) unless otherwise noted. JWH-015, and JWH-200 were from Cayman Chemical (Ann Arbor, MI). JWH-120, JWH-148, and JWH-042 were a kind gift from John W. Huffman (Clemson University, Clemson, SC). ST compounds were synthesized in house as described (14 , 15 ). Z-DEVD-FMK was from Santa Cruz Biotechnology (Santa Cruz, CA). L-α-Phosphatidylcholine (EPC) was from Avanti Polar Lipids (Alabastar, AL). 1,2-Dimyristoyl-sn-glycero-3-phosphoglycerol, sodium salt (DMPG) and N-(Carbonyl-methosypolyethyleneglycol-2000)-1,2-dimyristoyl-sn-glycero-3-phosphoethanolzmine, sodium salt (DMPE-mPEG2000) were from Corden Pharma (Plankstadt, Germany). Cell culture materials were purchased from Life Technologies (Thermo Fisher Scientific, Waltham, MA).
Cherry A.E., Haas B.R., Naydenov A.V., Fung S., Xu C., Swinney K., Wagenbach M., Freeling J., Canton D.A., Coy J., Horne E.A., Rickman B., Vicente J.J., Scott J.D., Ho R.J., Liggitt D., Wordeman L, & Stella N. (2016). ST-11: a new brain-penetrant microtubule-destabilizing agent with therapeutic potential for glioblastoma multiforme. Molecular cancer therapeutics, 15(9), 2018-2029.
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