S2020

Manufactured by Agilent Technologies

The S2020 is a laboratory equipment product from Agilent Technologies. It is designed for analytical testing and measurement applications. The core function of the S2020 is to provide precise and reliable data collection and analysis capabilities for scientific research and industrial processes.

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Lab products found in correlation

3 protocols using s2020

Immunolocalization of NeuGcGM3 Ganglioside

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Five-micrometer serial sections from each block were obtained in a micrometer (Leitz, 1512) and they were mounted on plus slides (Dako, S2024). All sections were attached to the slide by heating in a 60°C oven for 1 h. Afterward, the slides were dewaxed in xylene and rehydrated in graded ethanol series in the usual way. The samples were maintained in tap water until they were stained.
The immunolocalization of NeuGcGM3 ganglioside was performed as it was previously described in [10 (link)] with some modifications. Briefly, the slides were incubated with 14F7 Mab in a humid chamber for 1 h at room temperature followed by the labeled streptavidin biotin (LSAB) two steps' system (Dako, K0690) both for 30 minutes at room temperature. The enzymatic activity was visualized with 3,3-diaminobenzidine (DAB) substrate chromogenic solution (Dako, K3465) and the tissues were counterstained with Mayer's Hematoxylin (Dako, S2020). Concerning the evaluation of both EGFR and EGF tissue antigens, the procedure as it was previously described in [19 (link)] was used.

Blanco R., Domínguez E., Morales O., Blanco D., Martínez D., Rengifo C.E., Viada C., Cedeño M., Rengifo E, & Carr A. (2015). Prognostic Significance of N-Glycolyl GM3 Ganglioside Expression in Non-Small Cell Lung Carcinoma Patients: New Evidences. Pathology Research International, 2015, 132326.

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TERT Expression in Melanoma Immunohistochemistry

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Immunohistochemical staining was performed on 5 μm TMA sections. Sufficient tumour tissue for immunohistochemistry was available in 248 of the 255 primary melanoma cases and 68 of the 78 paired loco-regional metastatic melanomas. For detection of TERT expression, the slides were dewaxed with xylene/ethanol before microwave antigen retrieval for 30 min in Target Retrieval Solution (DAKO 1699, Glostrup, Denmark) (pH 6). Endogenous peroxidase activity was prevented by treating the slides with peroxidase block (DAKO S2001) for 8 min. The slides were incubated with an anti-telomerase catalytic subunit (hTERT) polyclonal rabbit antibody (dilution 1:125) (catalogue number 600-401-252) (Rockland, Limerick, PA, USA) overnight at 4 °C. The reliability of this antibody has been validated in the study by Wu et al (2006) (link). The staining procedure was performed using the anti-rabbit EnVision labelled polymer method (DAKO K4011) with 3-amino-9-ethylcarbazole (DAKO K3469) as substrate chromogen. Brief counterstaining was performed with haematoxylin (DAKO S2020). Negative controls were obtained by omitting the primary antibody.

Hugdahl E., Kalvenes M.B., Mannelqvist M., Ladstein R.G, & Akslen L.A. (2017). Prognostic impact and concordance of TERT promoter mutation and protein expression in matched primary and metastatic cutaneous melanoma. British Journal of Cancer, 118(1), 98-105.

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Immunohistochemical Analysis of uPAR in Melanoma

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The immunohistochemical staining was performed on 5 μm TMA sections of paraffin-embedded archival tissue. Sufficient tumor tissue for immunohistochemistry was available in 248 of the primary melanoma cases and 68 of the loco-regional metastatic melanomas. The slides were dewaxed with xylene/ethanol. Antigen retrieval was performed for 20 minutes in Target Retrieval Solution (DAKO 1699) (pH = 6) in microwave. Endogenous peroxidase activity was prevented by treating the slides with peroxidase block (DAKO S2001) for 8 minutes. The slides were incubated with the mouse monoclonal antibody uPAR (dilution 1:100) (ADG 3937, Sekisui Diagnostics, Pfungstadt, Germany) overnight at 4°C. EnVision labelled polymer method was then used (DAKO K4001 or K4003) for 30 minutes. 3-amino-9-ethylcarbazole (AEC) (DAKO K3469) was used as substrate chromogen. Brief counterstaining was performed with hematoxylin (DAKO S2020). Negative control was obtained by omitting the primary antibody.

Hugdahl E., Bachmann I.M., Schuster C., Ladstein R.G, & Akslen L.A. (2019). Prognostic value of uPAR expression and angiogenesis in primary and metastatic melanoma. PLoS ONE, 14(1), e0210399.

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