Fluorescent polystyrene beads 0.2 μm in size (FluoSpheres sulfate microspheres F8848, carboxylate-modified microspheres F8811, amine-modified microsphere F8764; Thermo Fisher) were diluted 300 times to 0.02% (wt/vol) in BG11 and used for the bead assay, as previously described (Nakane and Nishizaka, 2017 (link)). A coverslip was coated with 0.2% (vol/vol) collodion in isoamyl acetate and air-dried before use. The cell culture was poured into a tunnel chamber assembled by taping a coverslip. After incubation at 45°C for 2 min on the microscope stage, the cells were subjected to vertical illumination from blue-light LED through a dark-field condenser at a fluence rate of 200 μmol m−2 s−1. After illumination for 2 min, fluorescent beads were added to the sample chamber, and their movement was visualized by blue light illumination at 0.1-s intervals. Lateral illumination from green-light LEDs was applied for 2 min before adding the beads if needed.
Fluospheres sulfate microspheres f8848
Manufactured by Thermo Fisher Scientific
FluoSpheres sulfate microspheres F8848 are monodisperse fluorescent microspheres that provide a uniform and reproducible fluorescent signal. They are available in a range of excitation and emission wavelengths and can be used for various applications, such as flow cytometry, fluorescence microscopy, and cell labeling.
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Lab products found in correlation
2 protocols using fluospheres sulfate microspheres f8848
1
Fluorescent Bead Motility Assay
2
Fluorescent Bead Assay for Cell Motility
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Fluorescent polystyrene beads 0.2 μm in size (FluoSpheres sulfate microspheres F8848, carboxylate-modified microspheres F8811, amine-modified microsphere F8764; Thermo Fisher) were diluted 300 times to 0.02% (wt/vol) in BG11 and used for the bead assay, as previously described (7) (link). A coverslip was coated with 0.2% (vol/vol) collodion in isoamyl acetate and air-dried before use. The cell culture was poured into a tunnel chamber assembled by taping a coverslip. After incubation at 45 °C for 2 min on the microscope stage, the cells were subjected to vertical illumination from blue-light LED through a dark-field condenser at a fluence rate of 200 μmol m -2 s -1 . After illumination for 2 min, fluorescent beads were added to the sample chamber, and their movement was visualized by blue light illumination at 0.1-s intervals. Lateral illumination from green-light LEDs was applied for 2 min before adding the beads if needed.
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