Western blot analyses were performed on protein extracts from CMs, ECs and VSMCs as previously described [17 (link)]. The antibodies used were, SV40 TAg and IκBα from Santa Cruz Biotechnology, p53 and TnT from Calbiochem, phospho-AKT (T308), AKT, p-p70S6K (Ser389), p70S6K, p-AMPK (T172), AMPK, p-JNK-1/2 (T183/Y185), Cleaved Caspase 3, p-p42/44 (S202/T204) and p42/44 from Cell Signalling, p-IRS-1 from Millipore and p-Tyr, PAI-1, α- and β-actin and α-tubulin from Sigma Corp.
P jnk 1 2 t183 y185
Manufactured by Cell Signaling Technology
Sourced in United States
P-JNK-1/2 (T183/Y185) is a lab equipment product that detects the phosphorylation of c-Jun N-terminal kinase (JNK) at threonine 183 and tyrosine 185 residues. This phosphorylation is a key activation event in the JNK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
P irs 1, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
γ 32p atp, by Cytiva (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Antibody against β actin, by Merck Group (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Glutathione sepharose 4b, by Cytiva (1 mentions)
Chemiluminescence detection kit, by Cytiva (1 mentions)
Jnk1 2, by Cell Signaling Technology (1 mentions)
Prb s780, by Cell Signaling Technology (1 mentions)
P gsk3β s9, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Cyclin e, by Cell Signaling Technology (1 mentions)
Cnbr sepharose 4b, by Cytiva (1 mentions)
2 protocols using p jnk 1 2 t183 y185
1
Comprehensive Protein Expression Analysis
2
Signaling Pathway Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RPMI 1640 medium, basal medium eagle (BME), gentamicin, and L-glutamine were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against p-Akt (S473), p-PTEN (S380), PTEN, p-ERK1/2 (T202/Y204), ERK, p-p90RSK (T359/S363), p90RSK, p-JNK1/2 (T183/Y185), JNK1/2, p-GSK3β (S9), GSK3β, cyclin D1, CDK4, cyclin E, p-CDK2 (Y15), CDK2, and p-RB (S780) were obtained from Cell Signaling Biotechnology (Beverly, MA, USA). Antibodies against p85 and p110 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibody against β-actin was obtained from Sigma-Aldrich (St. Louis, MO, USA) and the active PI3K protein was obtained from EMD Millipore (Billerica, MA, USA), CNBr-Sepharose 4B, glutathione-Sepharose 4B, [γ-32P] ATP and the chemiluminescence detection kit were purchased from Amersham Pharmacia Biotech (Piscataway, NJ, USA), and the protein assay kit was from Bio-Rad Laboratories (Hercules, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!