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Hrp conjugated anti mouse igg

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HRP-conjugated anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP). This conjugate can be used to detect and quantify mouse IgG in various immunoassays, such as ELISA, Western blotting, and immunohistochemistry.

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Lab products found in correlation

Hdac2, by Merck Group (2 mentions) Donkey anti rabbit igg, by Avantor (2 mentions) Histone h4, by Cell Signaling Technology (2 mentions) Nuclear complex co ip kit, by Active Motif (2 mentions) Halt phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Hrp conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Anti flag m2 antibody, by Merck Group (1 mentions)

3 protocols using hrp conjugated anti mouse igg

1

Immunoblotting Procedure with Quantification

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For immunoblotting, cells were lysed in RIPA lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1% Igepal, 0.5% deoxycholate, 0.1% SDS) containing 1× HALT phosphatase inhibitor cocktail (Thermo Scientific, Waltham, MA) and a 1× complete protease inhibitor cocktail (Roche). Cell lysis, SDS-PAGE, and immunoblotting were carried out as described.4 (link) NF2/merlin polyclonal C26 antibody was diluted at 1:200 in 1% milk/TBS-T (TRIS-buffered saline with 2% Tween 20), as described.18 (link) Antibodies for phospho-S6 (S240/244) and S6 (Cell Signaling Technology, Danvers, MA) were diluted at 1:1,000 in 5% BSA/TBS-T. Anti-FLAG M2 antibody (Sigma) was diluted at 1:2,000 in 2% milk/TBS-T. Secondary antibodies included HRP-conjugated anti-rabbit IgG (Cell Signaling Technology) and HRP-conjugated anti-mouse IgG (VWR International, Radnor, PA). The quantitation of immunoblotting was carried out using ImageJ/Fiji open source image processing and analysis software.51 (link)
Prabhakar S., Beauchamp R.L., Cheah P.S., Yoshinaga A., Haidar E.A., Lule S., Mani G., Maalouf K., Stemmer-Rachamimov A., Jung D.H., Welling D.B., Giovannini M., Plotkin S.R., Maguire C.A., Ramesh V, & Breakefield X.O. (2022). Gene replacement therapy in a schwannoma mouse model of neurofibromatosis type 2. Molecular Therapy. Methods & Clinical Development, 26, 169-180.
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2

Epigenetic Protein Complex Profiling

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Nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif). Proteins were separated using SDS-PAGE, transferred to PVDF membranes, and probed with antibodies to EZH2 (5246, Millipore), HDAC2 (05–814, Millipore), NSD2 (39 (link)), and histone H4 (2935, Cell Signaling Tech.). Secondary antibodies were HRP-conjugated anti-mouse IgG (95017–332, VWR) or donkey anti-rabbit IgG (95017–556, VWR).
Swaroop A., Oyer J.A., Will C.M., Huang X., Yu W., Troche C., Bulic M., Durham B.H., Wen Q.J., Crispino J.D., MacKerell AD J.r., Bennett R.L., Kelleher N.L, & Licht J.D. (2018). An Activating Mutation of the NSD2 Histone Methyltransferase Drives Oncogenic Reprogramming in Acute Lymphocytic Leukemia. Oncogene, 38(5), 671-686.
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3

Epigenetic Protein Complex Profiling

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Nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif). Proteins were separated using SDS-PAGE, transferred to PVDF membranes, and probed with antibodies to EZH2 (5246, Millipore), HDAC2 (05–814, Millipore), NSD2 (39 (link)), and histone H4 (2935, Cell Signaling Tech.). Secondary antibodies were HRP-conjugated anti-mouse IgG (95017–332, VWR) or donkey anti-rabbit IgG (95017–556, VWR).
Swaroop A., Oyer J.A., Will C.M., Huang X., Yu W., Troche C., Bulic M., Durham B.H., Wen Q.J., Crispino J.D., MacKerell AD J.r., Bennett R.L., Kelleher N.L, & Licht J.D. (2018). An Activating Mutation of the NSD2 Histone Methyltransferase Drives Oncogenic Reprogramming in Acute Lymphocytic Leukemia. Oncogene, 38(5), 671-686.
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