Cell migration was measured by a Transwell filter system (Corning-Costar, New York, US) with a filter with 8 µm pores. After culture in serum-free medium for 24 h, the cells (2×105 cells/well, 300 µL) of the different groups were seeded into the upper chamber of the insert, and a complete medium (700 µL) was added to the lower chamber. The Transwell filter systems were then incubated for 72 h at 37°C with 5% CO2. The cells were fixed with methanol (Sinpharm, Beijing, China), and the cells that remained on the upper surface of the filter were removed carefully with a cotton swab. The cells were stained with 0.5% crystal violet (Solarbio, Beijing, China) for 30 mins and counted under a microscope (Caikon, Shanghai, China).
Cell scratch test can also be used to examine cell migration. After culture for 24 h, the cells (8×105 cells/well) of the different groups were seeded into the culture medium. A 10 µL pipette tip was used to draw a line in the middle of the dish, and the cells were cultured in an incubator for 24 h. Pictures of the scratches were taken at 0 h and 24 h.
Cell scratch test can also be used to examine cell migration. After culture for 24 h, the cells (8×105 cells/well) of the different groups were seeded into the culture medium. A 10 µL pipette tip was used to draw a line in the middle of the dish, and the cells were cultured in an incubator for 24 h. Pictures of the scratches were taken at 0 h and 24 h.