Anti pd 1

Manufactured by Miltenyi Biotec

Sourced in France

Anti-PD-1 is a lab equipment product from Miltenyi Biotec. It functions as an antibody that binds to the PD-1 protein, which is involved in regulating the immune response.

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Lab products found in correlation

Gentlemax, by Miltenyi Biotec (1 mentions) Anti hla dr, by BD (1 mentions) Anti hla dr, by Diaclone (1 mentions) Anti tim 3, by Miltenyi Biotec (1 mentions) Anti pd l1, by BD (1 mentions) Percoll, by Merck Group (1 mentions) Facscanto 2, by BD (1 mentions) Ficoll, by Harvard Bioscience (1 mentions) Opteia human ifn gamma elisa set, by BD (1 mentions) Microwin software, by Berthold Technologies (1 mentions) Rpmi 1640, by Lonza (1 mentions) Ultraglutamine, by Lonza (1 mentions) Penicillin, by Lonza (1 mentions) Streptomycin, by Lonza (1 mentions) Fetal calf serum (fcs), by Lonza (1 mentions)

2 protocols using anti pd 1

MHC and Immune Checkpoint Profiling

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For the analysis of MHC molecules expression by SARC-L1, cells were stained with the
following fluorescent conjugated monoclonal antibodies: Anti-2K^b, (clone
AF6-88.5.5.3,); anti-H-2K^dand anti-I^A/I^E(Ebiosciences, France); anti-HLA-A2 (BD Biosciences, France); anti-HLA-DR (Diaclone,
France) or with their respectively isotype control. For tumor infiltrating immune
cells analysis, tumor cells were mechanically dissociated (GentleMax, Miltenyi,
France) tumor infiltrating lymphocytes were separated on a Percoll (Sigma-Aldrich,
France) density gradient. Lymphocytes layer was collected and stained with the
following fluorescent conjugated monoclonal antibodies: anti-CD3, anti-CD4, anti-
CD8, anti-Gr1, anti-PD-L1 (Biolegend, France), anti-CD11b, anti- FoxP₃,
anti-CD25 (eBiosciences, France), anti-PD-1, and anti-TIM-3 (Miltenyi, France),
fixable viability stain (BD, France). The tumor cells fraction was stained
withanti-CD45, anti-PD-L1, anti-HLA-A2 and anti-HLA-DR (BD Biosciences, France) and
fixable viability stain (Biolegend, France). Samples were acquired on a FACS Canto II
(BD Biosciences, France) and analyzed with the BDFacsDIVA or FlowJo software.

Rangan L., Galaine J., Boidot R., Hamieh M., Dosset M., Francoual J., Beziaud L., Pallandre J.R., Lauret Marie Joseph E., Asgarova A., Borg C., Al Saati T., Godet Y., Latouche J.B., Valmary-Degano S, & Adotévi O. (2017). Identification of a novel PD-L1 positive solid tumor transplantable in HLA-A*0201/DRB1*0101 transgenic mice. Oncotarget, 8(30), 48959-48971.

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Stimulation of PBMC for IFN-gamma production

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Buffy coats from anonymized healthy donors were obtained from the “DRK-Blutspendedienst – Ost”. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll (Biochrom). Cells were cultured in complete medium (RPMI1640 [Lonza] with 2 mM UltraGlutamine [Lonza] supplemented with 10% [v/v] fetal calf serum [Linaris], 100 U/ml Penicillin and 100 µg/ml Streptomycin [Lonza]) in flat-bottom plates (PBMC, 6 million cells/ml). Cells were stimulated with 1 µg/ml extended CEF (cytomegalo virus, Epstein-Barr virus, influenza virus) peptide pool (JPT Peptide Technologies GmbH), 3 µM lefitolimod and 10 µg/ml anti-PD-1 (Miltenyi Biotec) for 2 days. IFN-gamma in the culture supernatant was determined by ELISA (OptEIA Human IFN gamma ELISA Set, BD Biosciences) and was performed in duplicates according to the manufacturer’s instructions. Optical density was measured at 450 nm; the data were analyzed with the MicroWin software (Berthold Technologies).

Kapp K., Volz B., Oswald D., Wittig B., Baumann M, & Schmidt M. (2019). Beneficial modulation of the tumor microenvironment and generation of anti-tumor responses by TLR9 agonist lefitolimod alone and in combination with checkpoint inhibitors. Oncoimmunology, 8(12), e1659096.

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