Anti ho 1 ab68477

LPS (Escherichia. coli O127) was purchased from Fujifilm Wako Pure Chemicals (Osaka, Japan). Anti-Lamin B1 (ab16048), anti-Nrf2 (ab2352), and anti-HO-1 (ab68477) were from Abcam (Cambridge, UK), and anti-NF-κB (#8242), anti-β-Actin (#4967) and anti-rabbit IgG-HRP (#7074) were purchased from Cell Signaling Technology (Danvers, MA). Nrf2 inhibitor ML385 was purchased from Selleck (Houston, TX, USA).

FZHY was obtained from Huanghai Pharmaceutical Co. (Shanghai, China). RPMI 1640 medium, fetal bovine serum (FBS), and 0.25% trypsin were purchased from Gibco (Rockville, MD, USA). BCA protein assay kit and TRIzol reagent were purchased from Invitrogen (Carlsbad, CA, USA). Special antibodies including p-JNK(#9251; 1 : 1000), T-JNK(#9252; 1 : 1000), p-ERK(#9101; 1 : 1000), T-ERK(#9102; 1 : 1000), p-P38(#9211; 1 : 1000), T-P38(#9212; 1 : 1000), p-STAT-1(#7649; 1 : 1000), T-STAT-1(#14994; 1 : 1000), and iNOS(#13120; 1 : 1000) were purchased from cell signaling technology (Beverly, MA, USA). Anti-HO-1 (ab68477; 1 : 10000), CD40 (ab252428; 1 : 1000), IL-6 (ab259341; 1 : 1000), and horseradish peroxidase-labeled goat antirabbit IgG were purchased from Abcam (Cambridge, UK). CD86 (13395-1-AP; 1 : 500) was purchased from ProteinTech Group (Chicago, IL, USA). Polyvinylidene difluoride (PVDF) membranes, western blotting detection reagent ECL, and murine recombinant IFN-γ were purchased from Millipore (Bedford, MA, USA). LPS, 1400W, Griess reagent, and MTT were purchased from Sigma-Aldrich (St. Louis, MO, USA). MicroRNA primers, transcription kits, and universal PCR master mix were obtained from GenePharma Company (Shanghai, China). Standard compounds sodium danshensu, salvianolic acid B, and adenosine were purchased from the National Institutes for Food and Drug Control (Beijing, China).

Anti-NRF2 (sc-13032), anti-Lamin B (sc-6217), anti-Pol II (sc-899), anti-HSP90 (sc-59577) and anti-BACH1 (sc-14700) antibodies were purchased from Santa Cruz Biotechnology, Inc. Anti-β-Actin antibody (A1978) was obtained from Sigma-Aldrich. Anti-Pol II antibody (clone CTD4H8) and normal mouse immunoglobulin G (IgG) were purchased from Millipore. Anti-HO-1 (ab68477) was purchased from Abcam.