Fresh tilapia (average length: 30 ± 5 cm, weight: 1300 ± 20 g) was purchased from Dongfeng Market (Zhanjiang, China). Fish collagen peptide was purchased from Ruikanglai Technology Co., Ltd. (Nanjing, China); glucosamine hydrochloride was purchased from Cohen Biotechnology Co., Ltd. (Yangzhou, China); transglutaminase and bovine serum albumin were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China); sodium chloride, calcium chloride, and ethanol were purchased from Guanghua Technology Co., Ltd. (Guangdong, China); and SDS–PAGE electrophoresis solution was purchased from Biyuntian Biotechnology Co., Ltd. (Shanghai, China).
Sds page electrophoresis solution
Manufactured by Beyotime
Sourced in China
SDS-PAGE electrophoresis solution is a laboratory reagent used in the analysis of proteins. It is designed to facilitate the separation and identification of proteins based on their molecular weight through the process of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Yuanye Bio-Technology (1 mentions)
Transglutaminase, by Yuanye Bio-Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Ros kit, by Beyotime (1 mentions)
P0015, by Beyotime (1 mentions)
Ecl developer, by Thermo Fisher Scientific (1 mentions)
Trizol lysis buffer, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)
Tb green premix ex taq 2, by Takara Bio (1 mentions)
Sds page gel preparation kit, by Solarbio (1 mentions)
Bca protein concentration assay kit, by Beyotime (1 mentions)
D8340, by Solarbio (1 mentions)
Skimmed milk powder, by Solarbio (1 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Ham s f 12k medium, by Thermo Fisher Scientific (1 mentions)
Sds page gel preparation kit, by Beyotime (1 mentions)
Basement membrane matrix, by Corning (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Hsp90, by Cell Signaling Technology (1 mentions)
5 protocols using sds page electrophoresis solution
1
Tilapia Collagen Peptide Enrichment
2
Pea Oligosaccharides Extraction and Microbial Analysis
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Pea oligosaccharides were extracted from pea powder by ultrasonic-assisted water extraction under the conditions of 1:12 g/mL solid–liquid ratio, 30 s under 700 W microwave, 22 min under 190 W ultrasonic, and being decolorized by AB-8 macroporous resin; the components of the pea oligosaccharides are listed in Figure S1 in the Supplementary Materials .
Brain Heart Infusion Broth (BHI), Lactobacilli MRS agar, Bifidobacterium agar medium, Clostridium perfringens agar medium, and eosin-methylene blue agar were purchased from Haibor Biology (Qingdao, China). Lactic acid, acetic acid, propionic acid, butyric acid standard, DMEM high-glucose medium, trypsin, PBS, LPS, and loaded buffer Rainbow 245 broad-spectrum protein products were purchased from Soleibao (Beijing, China). RAW264.7 macrophages were purchased from Henan Academy of Agricultural Sciences (Zhengzhou, China). SDS-PAGE electrophoresis solution, BCA protein concentration determination box, and ROS kit were purchased from Biyuntian (Shanghai, China). MTT reagent was purchased from Punosei Bio (Wuhan, China). Fetal bovine serum (FBS) and DMSO were purchased from Gibco (Grand Island, NY, USA). TNF-α and IL-6 inflammatory cytokine kit were purchased from Shanghai Enzyme Link (Shanghai, China). NF-κB p65/p-p65 and iNOS antibody were purchased from Sanying (Wuhan, China).
Brain Heart Infusion Broth (BHI), Lactobacilli MRS agar, Bifidobacterium agar medium, Clostridium perfringens agar medium, and eosin-methylene blue agar were purchased from Haibor Biology (Qingdao, China). Lactic acid, acetic acid, propionic acid, butyric acid standard, DMEM high-glucose medium, trypsin, PBS, LPS, and loaded buffer Rainbow 245 broad-spectrum protein products were purchased from Soleibao (Beijing, China). RAW264.7 macrophages were purchased from Henan Academy of Agricultural Sciences (Zhengzhou, China). SDS-PAGE electrophoresis solution, BCA protein concentration determination box, and ROS kit were purchased from Biyuntian (Shanghai, China). MTT reagent was purchased from Punosei Bio (Wuhan, China). Fetal bovine serum (FBS) and DMSO were purchased from Gibco (Grand Island, NY, USA). TNF-α and IL-6 inflammatory cytokine kit were purchased from Shanghai Enzyme Link (Shanghai, China). NF-κB p65/p-p65 and iNOS antibody were purchased from Sanying (Wuhan, China).
3
Comprehensive Protein Analysis Protocol
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The main reagents used in this study included TRIzol lysis buffer (Invitrogen, USA); reverse transcription kit: PrimeScript RT reagent kit with GDNA Eraser (RR 047A, Takara, Japan); qPCR kit: Tb Green Premix EX TAQ II (RR 820A, Takara); animal whole protein extraction kit (C510003, Sangon Biotech, China); BCA protein concentration assay kit (P0010, Beyotime Biotech, China); 5× loading buffer (P0015, Beyotime Biotech); SDS-PAGE gel preparation kit (P1200, Solar BioBeijing, China); SDS-PAGE electrophoresis solution (P00148, Beyotime Biotech); 10× EMT (D1060, Solar BioBeijing); methanol (CB/T693-1993, Shanghai Guangnuo Chemical Technology Co., Ltd., China); skimmed milk powder (D8340, Solar BioBeijing); 10×TBST buffer (powder) (T1087, Solar BioBeijing); nitrocellulose membrane (NC membrane) (37412133, PALL, USA); and ECL developer (34095, Thermo Fisher, USA).
4
Optimized Cell Culture and Transfection
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Ham's F-12 K medium, 10 % Fetal Bovine Serum (FBS), 0.25 % Trypsin-EDTA, and Lipofectamine 3000 Transfection Reagent were purchased from Gibco (New York, USA). Basement Membrane Matrix was obtaine from Corning (New York, USA). Crystal Violet Staining Solution, SDS Page Gel Preparation Kit, BCA Protein Quantitation Kit, and SDS PAGE electrophoresis solution were bought from Beyotime (Shanghai, China). The primary antibodies against CD54, β-Catenin, c-Myc, LEF1 and HSP90 were purchased from Cell Signaling Technology (Massachusetts, USA). One Step PrimeScript RT-PCR Kit was bought from Takara (Tokyo, Japan).
5
Transwell Invasion Assay with Magnolol
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Dulbecco’s modified Eagle’s Medium (DMEM), Transwell nesting (with polycarbonate film 605 mm 8.0 μm), and Matrigel™ were purchased from Corning (NY, USA). Fetal bovine serum (FBS) was purchased from Tianhang (Huzhou, China). Dimethyl sulfoxide (DMSO) and 0.25% trypsin-EDTA (1X) were purchased from Sigma-Aldrich (Shanghai, China). The penicillin and streptomycin (100×) was purchased from Solarbio (Beijing, China). Magnolol and 5-FU were purchased from Selleck (Shanghai, China). Cell counting kit-8 (CCK-8), Phosphate buffer solution (PBS), Western transfer solution, SDS-PAGE electrophoresis solution and Crystal violet stain solution were purchased from Beyotime (Shanghai, China). iFluor 488-conjugated phalloidin was purchased from Abcam (Shanghai, China). First and second antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
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