Twenty-five-day-old plants grown in pots (length × width × height = 7.0 × 7.0 × 7.6 cm) were placed in trays and saturated either with 1/10 Hoagland solution (as control) or with 1/10 Hoagland solution containing 500 μM of CdCl2 solution for 10 days. Pots were then transferred to trays containing normal culture medium to promote plant recovery for 12–15 days, after which the plants’ fresh weight and height were measured. Cd content was also measured using an atomic absorption spectrometer (Model AA-6800, Shimadzu Corporation, Japan). The experiment was performed with three independent biological replicates.
Model aa 6800
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu Model AA-6800 is an atomic absorption spectrophotometer. It is designed for the analysis and determination of trace elements in various samples.
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7 protocols using model aa 6800
1
Cadmium Stress Response in Plants
2
Nutrient Analysis of Black-Eyed Pea and Plantain
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The raw dry black-eyed pea (bean) seeds (Vigna unguiculata L) (BS) and a bunch of unripe plantain fruits were purchased from Ogige market in Nsukka, Enugu State, Nigeria. Analytical grade chemicals, including potassium standard solution 1000 mg/L (Scharlau Chemie SA), 0.1 M HCl (Sigma-Aldrich Laborchemikalien, Germany) and NaOH (BDH Laboratory, Poole, England) were without further purification. Deionised water was used throughout the experiments. A digital pH meter (pHep, Hanna Instruments) was used to monitor the pH values during adjustment with either the acidic or basic solution. Atomic absorption spectrophotometer (AAS) (Model AA-6800, Shimadzu Corporation, Japan) was for determining elemental compositions.
3
Potassium Adsorption on Biosorbent
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Exactly 100 ml of 75 ppm potassium solution was added to 2 g of BS powder in different conical flasks. The pH of the solution in the flasks was adjusted with 0.1 M HCl and 0.1 M NaOH to obtain pH values of 3.9, 4.7, 6.5, 8.1 and 10.2 and measured with a pH meter. The solution in each flask was agitated with a magnetic stirrer for 180 min before filtering into a clean sample bottle. The filtrate was analysed for the concentration of potassium ion by atomic absorption spectrometry using AAS (Model AA-6800, Shimadzu Corporation, Japan). The optimum pH level, which is the pH that produced the highest adsorption, was noted [19 (link)].
4
Potassium Adsorption by Bean Seeds
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Potassium solutions of different concentrations (25, 50, 75, and 150 ppm) were prepared as described in section 2.2 . Respective 2.5, 5, 7.5 and 15 ml of the stock solution were transferred into 2 g of the powdered bean seeds in five separate 100 ml standard flasks. Each solution was adjusted to the optimum pH and agitated with a magnetic stirrer for 180 min before filtering into a clean sample bottle. The filtrate was analysed for the concentration of potassium ion adsorbed at different concentrations of potassium solutions by spectrometry using AAS (Model AA-6800, Shimadzu Corporation, Japan).
5
Potassium Adsorption on Boiler Slag
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Varying dosages (0.5, 1.0, 1.5 and 2 g) of the powdered BS were weighed into standard flasks. Exactly 100 ml of 75 ppm potassium solution was added to each of the flasks. Each solution was adjusted to the optimum pH and the solution was agitated with a magnetic stirrer for 180 min before filtering into a clean sample bottle. The filtrate was analysed for the concentration of potassium ion adsorbed at different dosages by spectrometry using AAS (Model AA-6800, Shimadzu Corporation, Japan) [19 (link)].
6
Adsorption Kinetics of Potassium from Aqueous Solution
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Exactly 100 ml of 75 ppm potassium solution was added to 2 g of BS powder in different standard flasks. The pH of the solution in the flasks was adjusted with 0.1 M HCl and 0.1 M NaOH to the optimum pH and the solutions were agitated with a magnetic stirrer at different contact time intervals (30, 90.120 and 180 min) before filtering into a clean sample bottle. The filtrates were analysed for the concentration of potassium adsorbed at different contact time by spectrometry using AAS (Model AA-6800, Shimadzu Corporation, Japan).
The adsorption behaviours of the adsorbent were studied from the percentage removal efficiency of potassium ion based on Equation(1) : Where Co is the initial concentration of potassium ion, Ce is the solution concentration after adsorption.
The adsorption behaviours of the adsorbent were studied from the percentage removal efficiency of potassium ion based on Equation
7
Trace Metal Analysis in Fish Samples
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The atomic absorption spectrophotometer (AAS) with a digital readout system (Model AA-6800, Shimadzu Corporation, Japan) was utilized to analyze the concentration of Cr, Fe, Cu, As, Cd and Pb in the digested solutions. For determining As concentrations, hydride vapor generators (HVG) were employed in conjunction with the flame AAS (FAAS) systems. The calibration standards for the instrument were prepared by diluting supplied standards (1000 mg/l) from Wako Pure Chemical Industry Ltd., Japan. To assess the method's accuracy, blanks and a certified reference material MA-A-2 [fish-flesh standard from International Atomic Energy Agency (IAEA), Vienna] were analyzed using the same procedure employed for fish samples. The mean recoveries of the analyzed metals ranged from 95% to 104%, demonstrating a favorable agreement between the certified and measured values.
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