GL21.T-miR34c chimera (4 μM) was incubated in 80% human serum for 1 to 96 hr. Type AB Human Serum provided by Euroclone (Cat. ECS0219D) was used. At each time point, 4 μL (16 pmol RNA) was withdrawn and incubated for 1 hr at 37°C with 3 μL of proteinase K solution (600 mAU/mL) in order to remove serum proteins that interfere with electrophoretic migration. Following proteinase K treatment, 9 μL TBE 1× and 2 μL gel loading buffer (Invitrogen, Waltham, MA, USA) were added to samples that were then stored at −80°C. All time point samples were separated by electrophoresis on 10% non-denaturing polyacrylamide gel. The gel was stained with ethidium bromide and visualized with a GEL.DOC XR (Bio-Rad) gel camera.
Type ab human serum
Manufactured by Euroclone
Sourced in Italy
Type AB Human Serum is a laboratory product derived from the blood of individuals with the AB blood type. It is a complex mixture of proteins, electrolytes, and other biomolecules found in human blood serum. This product can be used in various in vitro applications that require a source of human-derived serum components.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using type ab human serum
1
Serum Stability Assay for miRNA
2
Axl-148b Conjugate Stability in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conjugate stability was investigated by incubating the molecule in human serum (Type AB Human Serum, Euroclone Cat.ECS0219D) as in 25 (link). Briefly, Axl-148b was incubated in 80% human serum for 1 h to 7 days. At each time point 8μl of 80% serum solution (equal to 32 pmol RNA) was withdrawn and incubated for 2 h at 37 °C with 1μl of proteinase K solution (600 mAU/ml). Then, 9μl of 1×TBE and 3μl of gel loading buffer (Invitrogen, Waltham, MA, USA) were added to samples and stored at -80 °C. All time-point samples were separated by electrophoresis into 10% non-denaturing polyacrylamide gel stained with ethidium bromide.
3
MA 39/76 Aptamer Serum Stability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MA 39/76 aptamer was incubated at 4 μM concentration in 80% human serum (Type AB Human Serum provided by Euroclone, Milan, Italy) for 168 h. At each time point (from 1 h to 168 h), 16 pmol of samples were recovered and incubated for 1 h at 37°C with 5 μL of proteinase K solution (600 mAU/mL) to minimize serum proteins that impair electrophoretic migration. Following the addition of 18 μL of RNA dye (Invitrogen, Waltham, MA, United States), samples were kept at −80°C until subsequent analysis. All time point samples were loaded into a 15% polyacrylamide/urea 7 M denaturing gel and separated by electrophoresis. The gel was stained with ethidium bromide and exposed to UV light.
4
RNA Stability in Human Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gint4.T-STAT3 conjugates were incubated at 4 μM in 80% human serum (Type AB Human Serum provided by Euroclone) for increasing time points (from 1 hr to 72 hr). At each time point, 4 μL (16 pmoles RNA) was recovered and incubated for 1 hr at 37°C with 0.5 μL of proteinase K solution (600 mAU/mL) in order to remove serum proteins. Then, 4.5 μL 1 × Tris-borate-EDTA (TBE) and 1 μL gel loading buffer (Invitrogen, Waltham, MA, USA) were added to each sample before storing at −80°C. All time point samples were loaded on 10% non-denaturing polyacrylamide gel. The gel was visualized by UV exposure after ethidium bromide staining.
5
Serum Stability Analysis of Aptamers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To check serum stability, the aptamer was incubated at 4 μM in 85% human serum (Type AB Human Serum, Euroclone) from 1 to 96 h. At each time point, RNA (16 pmol) was recovered, and to remove serum proteins, samples were incubated for 1 h at 37°C with 2 μL of Proteinase K solution (600 mAU/mL). Then, electrophoretic migration into 15% denaturing polyacrylamide gel was performed, the gel was stained with ethidium bromide, and was visualized by UV exposure. The intensity of the bands was quantified with the ImageJ (NIH) program.
6
Time-Dependent Serum Stability of 2'-F-RNA A40s
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2′-F-RNA A40s was incubated until 7 days in 90% human serum (type AB human serum, EuroClone) at a starting concentration of 4 μM. At each time point, A40s (16 pmol) was recovered and incubated for 1 h at 37°C with 0.5 μL of proteinase K solution (600 mAU/mL) in order to remove serum proteins, which interfere with electrophoretic migration. Then, 9 μL of the denaturing gel loading buffer (1× TBE, 95% formamide, 10 mM EDTA, and bromophenol blue) were added to each sample before storing at −80°C. All time point samples were loaded on 15% polyacrylamide/urea (7 M) denaturing gel. The gel was visualized by UV exposure after ethidium bromide staining.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!