Sections were incubated with the following primary antibodies: anti-GPR43 (rabbit polyclonal antibody; 1 : 100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-β-arrestin-2 (rabbit polyclonal antibody; 1 : 100 dilution; Santa Cruz Biotechnology), anti-NF-κBp65 (goat polyclonal antibody; 1 : 100 dilution; Santa Cruz Biotechnology), and anti-MCP-1 (rabbit monoclonal antibody; 1 : 100 dilution; CST, Danvers, MA, USA) overnight at 4°C. After sections were washed with PBS, they were incubated with horseradish peroxidase (HRP) or fluorescein isothiocyanate fluorescent dye-conjugated secondary antibodies (1 : 200 dilution; Beijing Biosynthesis Biotechnology, Beijing China) for 2 h at room temperature. For visualizing the signals of immunohistochemistry, sections were treated with peroxidase substrate 3,3-diaminobenzidine and counterstained with hematoxylin. Positive staining areas were evaluated by Image-Pro Plus 6.0 software.
Horseradish peroxidase hrp or fluorescein isothiocyanate fluorescent dye conjugated secondary antibodies
Manufactured by Bioss Antibodies
Sourced in China
Horseradish peroxidase (HRP) or fluorescein isothiocyanate fluorescent dye-conjugated secondary antibodies are commonly used in various immunoassay techniques. They serve as detection reagents, binding to primary antibodies to amplify or visualize the target analyte. HRP catalyzes a colorimetric reaction, while fluorescein isothiocyanate dye emits fluorescent signals, enabling sensitive and quantitative detection.
Automatically generated - may contain errors
2 protocols using horseradish peroxidase hrp or fluorescein isothiocyanate fluorescent dye conjugated secondary antibodies
1
Immunohistochemical Analysis of GPR43, β-arrestin-2, NF-κB, and MCP-1
2
Immunohistochemical Analysis of Cellular Proteins
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Sections were incubated with the following primary antibodies: anti-PanKbu (mouse polyclonal antibody; 1 : 200 dilution; Hangzhou Jing Jie biological Co., Ltd; China), anti-H3K9bu (mouse polyclonal antibody; 1 : 200 dilution; Hangzhou Jing Jie biological Co., Ltd; China), anti-Fn (rabbit polyclonal antibody; 1 : 200 dilution; Abcam; UK), and anti-P300 (rabbit polyclonal antibody; 1 : 200 dilution; Cell Signaling Technology; USA) overnight at 4°C. After sections were washed with PBS, they were incubated with horseradish peroxidase (HRP) or fluorescein isothiocyanate fluorescent dye-conjugated secondary antibodies (1 : 200 dilution; Beijing Biosynthesis Biotechnology; China) for 2 h at room temperature. For visualizing the signals of immunohistochemistry, sections were treated with peroxidase substrate 3,3-diaminobenzidine and counterstained with hematoxylin. Each photograph of the stained sections was scanned using a light microscope.
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