Mouse anti human igg

Manufactured by R&D Systems

Sourced in Germany

Mouse anti-human IgG is an antibody that binds to human immunoglobulin G (IgG) proteins. It is commonly used in various immunoassay and immunohistochemical techniques to detect and quantify the presence of human IgG in biological samples.

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Lab products found in correlation

Lps of e coli, by Merck Group (1 mentions) Tnf α, by R&D Systems (1 mentions) Elisa development kit, by R&D Systems (1 mentions)

3 protocols using mouse anti human igg

Monocyte-Fibroblast/RPE Cell Interaction

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The day before experiment, RPE cells were serum‐starved. A total of 100 000 freshly purified human monocytes were added to confluent fibroblasts or RPE cells in DMEM‐PS1%. Treatments were applied directly to the culture medium. To stimulate Mos, we used lipopolysaccharide (LPS) of E.Coli (Sigma‐Aldrich, 40 ng mL⁻¹) or apolipoprotein E (APOE isoform 3, Leinco Technologies, 10 μg ML⁻¹). In specific experiments, cells were incubated with the indicated recombinant human proteins (TNF‐α, IL‐6, and IL‐1β), obtained from R&D. Blocking goat anti‐human TNF‐α (R&D) and mouse anti‐human IgG (R&D) antibodies were used at 25 μg mL⁻¹. Cells were incubated at 37 °C for 24 h or more for further experimentation. At the end of coculture, cells were washed two times with PBS and fixed in 4% paraformaldehyde (PAF) for 10 min. Alternatively, supernatants were removed and RA1 or RIPA lysis buffer was added on cells to perform real‐time polymerase chain reaction (RT‐PCR) or Western blot assays, respectively.

Mathis T., Housset M., Eandi C., Beguier F., Touhami S., Reichman S., Augustin S., Gondouin P., Sahel J., Kodjikian L., Goureau O., Guillonneau X, & Sennlaub F. (2016). Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF‐α. Aging Cell, 16(1), 173-182.

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Melanoma Circulating Tumor Cell Detection

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For assessment of MAP kinase signaling activity, antibodies directed against total S6K (rabbit anti-human total S6K, Cell Signaling), pS6K (rabbit anti-human pS6K, Cell Signaling), total ERK (mouse anti-human total ERK, R&D), and pERK (mouse anti-human pERK, R&D) were utilized. For detection of melanoma circulating tumor cells, a previously published four biomarker approach to detect MelanA/MART-1 (mouse anti-human MART-1, Thermo Fisher), gp100/HMB45 (rabbit anti-human gp100, Thermo Fisher), EpCAM (mouse anti-human IgG, R&D), and EGFR (humanized mouse anti-human EGFR, Imclone) was utilized, which previously was shown to be more accurate for CTC quantitation than single EpCAM marker-based methods (22 (link)). Secondary anti-mouse or anti-rabbit IgG antibodies (R&D) were used for nanoparticle labeling. Melanoma cell lines used for in vitro validation studies include the BRAF wild type cell lines MeWo, JuSo, and CHL and the BRAF V600E mutant lines A375, SKMel28, and UACC. HeLa cells were used as a non-melanoma control cell line. All cell lines were obtained from American Type Culture Collection.

Gee M.S., Ghazani A.A., Haq R., Wargo J.A., Sebas M., Sullivan R.J., Lee H, & Weissleder R. (2016). Point of care assessment of melanoma tumor signaling and metastatic burden from μNMR analysis of tumor fine needle aspirates and peripheral blood. Nanomedicine : nanotechnology, biology, and medicine, 13(3), 821-828.

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CCL18 Regulation of FGF2 Secretion

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Fibroblasts were seeded at a density of 300.000 cells per well in a 6-well cell culture plate in Quantum 333 medium. Cells were allowed to attach overnight, and the medium was replaced with 1 mL of DMEM plus 10% FCS. Cells were either left non-stimulated or were stimulated with 10 ng/mL of CCL18. To block CCR6/CCL18 interaction, a blocking antibody against CCR6 (R&D systems, Wiesbaden, Germany, FRG) or an irrelevant antibody (mouse anti human IgG, R&D Systems) was added to a final concentration of 20 µg/mL in parallel cultures. After 24 h of culture, the supernatant was harvested and stored at −80 °C until FGF2 determination. FGF2 was measured using an ELISA development kit (R&D) and performed as suggested by the supplier.

Höhne K., Wagenknecht A., Maier C., Engelhard P., Goldmann T., Schließmann S.J., Plönes T., Trepel M., Eibel H., Müller-Quernheim J, & Zissel G. (2024). Pro-Fibrotic Effects of CCL18 on Human Lung Fibroblasts Are Mediated via CCR6. Cells, 13(3), 238.

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