Mass spectrometry analysis was performed on a linear trap quadropole (LTQ) Orbitrap XL Discovery (Thermo Fisher Scientific) equipped with a nanoelectrospray ion source (Thermo) coupled to a Dionex Ultimate 3000 nanoflow LC system (Thermo Fisher Scientific). LC-MS/MS was performed essentially as described131 (link), using a 160 minute multistep gradient of solvent A (5% ACN and 0.1% formic acid in milliQ-water) and B (80% ACN and 0.1% formic acid in milliQ-water).
Dionex ultimate 3000 nanoflow lc system
Manufactured by Thermo Fisher Scientific
The Dionex Ultimate 3000 nanoflow LC system is a liquid chromatography system designed for high-performance nano-scale separations. It features precise flow control, low internal volume, and compatibility with nano-flow capillary columns for the analysis of small sample volumes.
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Lab products found in correlation
2 protocols using dionex ultimate 3000 nanoflow lc system
1
Proteomic Analysis by LTQ Orbitrap MS
2
Mass Spectrometry Analysis of CSF
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To analyze CSF samples, a Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled to a Dionex Ultimate 3000 nanoflow LC system (Thermo Fisher Scientific) was used. This section is comprised of four parts. The first subsection describes the methods for in-gel trypsin digestion of protein and peptide extraction, the second subsection describes peptide separation using liquid chromatography, the third subsection describes parameters used for mass spectrometry analysis, and the fourth subsection describes the methods for database search to determine peptide identity.
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