Myoma discs were fixed in 10% formalin and embedded in paraffin blocks. All samples were cut into 6-μm-thick sections and stained using a fully automated Leica BOND-MAX staining robot (Leica Microsystems, Wetzlar, Germany) and a horseradish peroxidase–labeled dextran polymer method. As primary antibodies, we used monoclonal mouse anti-human pan-cytokeratin (0.7 μg/ml), Ki67 (0.8 μg/ml), CD45 (0.94 μg/ml), and CD8 (3 μg/ml; all from DAKO, Glostrup, Denmark).
Monoclonal mouse anti human pan cytokeratin
Manufactured by Agilent Technologies
Sourced in Denmark
Monoclonal mouse anti-human pan-cytokeratin is a laboratory reagent used for the detection and localization of cytokeratins in human tissues. It is a purified immunoglobulin that recognizes a broad spectrum of cytokeratin polypeptides expressed in various epithelial cells.
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Lab products found in correlation
2 protocols using monoclonal mouse anti human pan cytokeratin
1
Immunohistochemical Analysis of Myoma Samples
2
Immunohistochemical Staining of Tissues
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Samples were cut into 4-μm-thick sections and stained using a fully automated Leica BOND-MAX staining robot (Leica Microsystems, Wetzlar, Germany) and a horseradish peroxidase–labeled dextran polymer method. As for primary antibodies, we used monoclonal mouse anti-human pan-cytokeratin (0.7 μg/mL) and Ki67 (0.8 μg/mL), both from Dako (Dako, Glostrup, Denmark).
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