Bladder cancer cell lines (HT1376 and 5637) were obtained from American Type Culture Collection (Rockville, MD) and were used within 10 passages of cell authenticity confirmation by genetic profiling (Genetica DNA Laboratory Inc., Cincinnati, OH). 253J cells and nonmalignant bladder epithelial cells (UROTSA) were a kind gift from Dr. Colin Dinney (MD Anderson Cancer Center, Houston, TX, USA) and Dr. Donald Sens (University of North Dakota, Grand Forks, ND, USA), respectively. Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Atlanta, GA), antibiotics (gentamycin) and cell culture media were purchased from Invitrogen (Carlsbad, CA) or Sigma-Aldrich (St. Louis, MO). All cells were grown in RPMI 1640 containing 10% FBS. For stable down regulation of ARRB2, 253J cells were transfected with HuSH/shRNA Plasmid Panels (Origene). For over expression of ARRB2 cells were transfected with an ARRB2 plasmid (RC201168, Origene). The details of transfection and other routine procedures are described in Supplementary Materials and Methods .
Antibiotics gentamycin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Gentamycin is an antibiotic used in laboratory settings. It is a broad-spectrum antibiotic that inhibits the growth of various types of bacteria. Gentamycin functions by interfering with bacterial protein synthesis, which is essential for the survival and reproduction of the microorganisms.
Automatically generated - may contain errors
2 protocols using antibiotics gentamycin
1
Bladder Cancer Cell Lines and Transfection Protocols
2
Bladder Cancer Cell Lines Cultivation
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Bladder cancer cell lines (HT1376, T24 and 5637) were obtained from American Type Culture Collection (Rockville, MD, USA) and were used within 10 passages. The 253J cells and nonmalignant bladder epithelial cells (UROTSA) were a kind gift of Dr. Colin Dinney (MD Anderson Cancer Center, Houston, TX, USA) and Dr. Donald Sens (University of North Dakota, Grand Forks, ND, USA), respectively. All of the above-described cells were cultured in RPMI medium supplemented with 10% FBS and gentamicin and cultured in a humidified incubator with 5% CO2 at 37 °C. Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Atlanta, GA, USA), antibiotics (gentamycin) and cell culture media were purchased from Invitrogen (Carlsbad, CA, USA) or Sigma-Aldrich (St. Louis, MO, USA). For over expression of ARRB1 cells were transfected with an ARRB1 plasmid (SC303424, Origene, Rockville, MD, USA) using Lipofectamine 3000 (L3000001, Thermo Fisher Scientific, Waltham, MA, USA).
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