RNA was isolated from 15 paired normal and HNSCC tumor tissues (a subset of the total 33 paired tissues) and evaluated for quality and integrity (Bio-Rad Experion™ System) as done previously [Westbrook et al., 2014 (link)]. Whole genome transcript analysis was performed using Affymetrix 3000 7G System platform using Human GeneChip 1.0ST arrays. The Human GeneChip 1.0ST covers >30,000 coding transcripts, along with a small number of lincRNA transcripts. RNA samples were processed per manufacturers’ protocols using Ambion® Whole-Transcript (WT) Expression kit, and Affymetrix® GeneChip WT Terminal Labeling and Controls Reagent kit on Affymetrix equipment (Scanner 3000 7G System). Hybridized chips were automatically washed, stained and scanned at the UMMC Institutional Molecular and Genomics Core. Data obtained from these gene expression studies are deposited in the Gene Expression Omnibus (GEO) database (http://www.ncbi.nlm.nih.gov/geo/ ) with the GEO accession number GSE58911.
Genechip wt terminal labeling and controls reagent kit
Manufactured by Thermo Fisher Scientific
The GeneChip WT Terminal Labeling and Controls Reagent kit is a laboratory product designed for use with Affymetrix GeneChip technology. The kit provides the necessary reagents and controls to enable the terminal labeling of fragmented, single-stranded cDNA derived from total RNA samples.
Automatically generated - may contain errors
2 protocols using genechip wt terminal labeling and controls reagent kit
1
Whole Genome Transcriptome Analysis of HNSCC
2
Whole Transcriptome Analysis of miRNA-Depleted Cells
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RNA was isolated from KHOS miROFF n-miR-G665 and KHOS miROFF Control cells and evaluated for quality and integrity (Bio-Rad Experion™ System). Whole genome transcript analysis was performed using Affymetrix 3000 7 G System platform using Human GeneChip 2.0ST arrays covering >30,000 coding transcripts. RNA samples were processed per manufacturers’ protocols using Ambion Whole-Transcript (WT) Expression kit, and Affymetrix GeneChip WT Terminal Labeling and Controls Reagent kit on Affymetrix equipment (Scanner 3000 7 G System). Hybridized chips processed at the UMMC Institutional Molecular and Genomics Core.
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