Ab56493

Protein expression of LIPG, ISG15 and DTX3L was examined by western blotting using a mouse antibody against LIPG (Abcam, ab56493) (RRID:AB_943999) and rabbit antibodies against ISG15 (Cell Signaling Technology, #2758 and #2743) (RRID:AB_2126200 and RRID:AB_2126201) and DTX3L (Bethyl Laboratories, Inc, A300-834A) (RRID: AB_2277461). Protein expression was detected by chemiluminescence (ECL, Pierce). Expression of α-tubulin (Thermo Fisher Scientific) (RRID:AB_2533035) was used as a protein loading control. Western blot data were quantified by densitometric analysis of autoradiograms, using a computerized densitometer (Typhoon System; Molecular Dynamics, Inc., Sunnyvale, CA). The quantitative protein level data were normalized by the α-tubulin protein levels.

Western blot analysis was performed as previously described^{16 (link)}. Protein expression of LIPG was examined by western blotting using a mouse antibody against LIPG (Abcam, ab56493). Protein expression was detected by chemiluminescence (ECL, Pierce). Expression of α-tubulin (Thermo Fisher Scientific) was used as a protein loading control. Western blot data were quantified by densitometric analysis of autoradiograms, using a computerized densitometer (Typhoon System; Molecular Dynamics, Inc., Sunnyvale, CA). The quantitative protein level data were normalized by the α-tubulin protein levels.