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Anti il 2 mq1 17h12

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Anti-IL-2 [MQ1-17H12] is a monoclonal antibody that binds to interleukin-2 (IL-2). It is commonly used in research applications to detect and quantify IL-2 levels.

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Lab products found in correlation

Anti cd3 sp34 2, by BD (2 mentions) Anti cd95 dx2, by BD (2 mentions) Anti ifn γ 4s b3, by BioLegend (2 mentions) Anti tnf α mab11, by BD (2 mentions) Anti cd28 cd28.2, by Beckman Coulter (2 mentions) Anti cd8 2st8.5h7, by Bio-Rad (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti perforin, by Mabtech (1 mentions) Anti pd 1 eh12.2h7, by BioLegend (1 mentions) Dextramer, by Immudex (1 mentions) Anti tnfα mab11, by BioLegend (1 mentions)

3 protocols using anti il 2 mq1 17h12

1

Multiparametric Flow Cytometry Immunophenotyping

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Surface stain monoclonal antibodies (mAbs) include: anti-CD4 [L200], anti-CD49d α4 integrin [9F10] and anti-CD95 [DX2] (BD Biosciences, San Jose, CA); anti-CD14 [TUK4], anti-CD20 [HI47] and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Grand Island, NY); anti-CD28 [CD28.2] (Beckman Coulter, Pasadena, CA); anti-CD8 [2ST8.5H7] (Custom, mAb from Serotec, conjugation kit from Invitrogen).Intracellular stain mAbs include: anti-CD3 [SP34-2] and anti-TNF-α [MAb11] (BD Biosciences); anti-IFN-γ [4S.B3] and anti-IL-2 [MQ1-17H12] (Biolegend, San Diego CA).
Kutzler M.A., Wise M.C., Hutnick N.A., Moldoveanu Z., Hunter M., Reuter M., Yuan S., Yan J., Ginsberg A., Sylvester A., Pahar B., Carnathan D., Kathuria N., Khan A.S., Montefiori D., Sardesai N.Y., Betts M.R., Mestecky J., Marx P, & Weiner D.B. (2015). Chemokine Adjuvanted Electroporated-DNA Vaccine Induces Substantial Protection from Simian Immunodeficiency Virus Vaginal Challenge. Mucosal immunology, 9(1), 13-23.
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2

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface stain monoclonal antibodies (mAbs) include: anti-CD4 [L200], anti-CD49d α4 integrin [9F10] and anti-CD95 [DX2] (BD Biosciences, San Jose, CA); anti-CD14 [TUK4], anti-CD20 [HI47] and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Grand Island, NY); anti-CD28 [CD28.2] (Beckman Coulter, Pasadena, CA); anti-CD8 [2ST8.5H7] (Custom, mAb from Serotec, conjugation kit from Invitrogen).Intracellular stain mAbs include: anti-CD3 [SP34-2] and anti-TNF-α [MAb11] (BD Biosciences); anti-IFN-γ [4S.B3] and anti-IL-2 [MQ1-17H12] (Biolegend, San Diego CA).
Kutzler M.A., Wise M.C., Hutnick N.A., Moldoveanu Z., Hunter M., Reuter M., Yuan S., Yan J., Ginsberg A., Sylvester A., Pahar B., Carnathan D., Kathuria N., Khan A.S., Montefiori D., Sardesai N.Y., Betts M.R., Mestecky J., Marx P, & Weiner D.B. (2015). Chemokine Adjuvanted Electroporated-DNA Vaccine Induces Substantial Protection from Simian Immunodeficiency Virus Vaginal Challenge. Mucosal immunology, 9(1), 13-23.
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3

Multiparametric Immune Cell Profiling

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The following mAbs were used: anti-CD6 (MT-605), anti-CD4 (L200), anti-CD8 (RPA-T8), anti-CD3 (SP34.2), anti-LAG-3 (T47-530), anti-IFN-γ (B27), anti-granzyme B (GB11), and anti-CD45 (D058-1283) (all from BD Bioscience, San Jose, CA); anti-perforin (MABTECH, Cincinnati, OH); anti-PD-1 (EH12.2H7), anti-IL-2 (MQ1-17H12) and anti-TNF-α (MAB11) (all from Biolegend, San Diego, CA); anti-CD206 (19.2) (eBioscience, San Diego, CA); polyclonal rabbit anti-SHP2 (Lifespan Biosciences, Seattle, WA); anti-CD43 (4-29-5-10-21), rabbit IgG isotype control and goat anti-rabbit alexa-488 (Invitrogen, Carlsbad, CA); Control immunoglobulin (mIgG1) was obtained from Sigma-Aldrich (St. Louis, MO). Purified anti-human/monkey TNF-α mAb (MT21A8), biotinylated anti-human/monkey TNF-α mAb (MT15B15), streptavidin-HRP and human TNF-α standard recombinant were obtained from Mabtech (Cincinnati, OH). CM9 (Gag, CTPYDINQM; residues 181–189 FITC) dextramer was obtained from Immudex (Fairfax, VA).
Enyindah-Asonye G., Nwankwo A., Rahman M.A., Hunegnaw R., Hogge C., Helmold Hait S., Ko E.J., Hoang T, & Robert-Guroff M. (2020). Overexpression of CD6 and PD-1 Identifies Dysfunctional CD8+ T-Cells During Chronic SIV Infection of Rhesus Macaques. Frontiers in Immunology, 10, 3005.
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