Danofloxacin

CYA, bi-deoxy Cyadox (Cy1), N4-deoxy cyadox (Cy2), N1-deoxy cyadox (Cy10), QCT, carbonyl reduction bi-deoxy quinocetone (Q2), MEQ, bi-deoxy Mequindox (M1), carbonyl reduction N1-deoxy mequindox (M4), carbonyl reduction bi-deoxy mequindox (M5) and carbonyl reduction mequindox (M6) (Table 1) were provided by the National Reference Laboratory of Veterinary Drug Residues, Huazhong Agricultural University (Wuhan, Hubei, China). Amphotericin B, tetracycline, doxycycline, ketoconazole, enrofloxacin, danofloxacin, rifampicin, tilmicosin and kitasamycin were purchased from Dr. Ehrenstorfer (Augsburg, Germany). Kanamycin, pyrazinamide, lincomycin, ethambutol, ribavirin and isoniazid were purchased from TRC (Toront, Canada). Amikacin, clindamycin, and tylosin were purchased from Sigma (St Louis, MO, USA). Stock solutions of the above compounds were prepared at a final concentration of 1280 μg/mL.Table 1

Chemical information of QdNOs and their metabolites

GSH, cadmium chloride hydrate (CdCl₂), tellurium powder, sodium borohydride (NaBH₄), NaOH, Bovine serum albumin (BSA), and ovalbumin (OVA), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), HEPES, NC membrane (Millipore HF90s) were purchased from Merck KGaA, (Darmstadt, Germany). MnO₂ NSs were purchased from Nanjing XFNANO Materials Tech Co., Ltd. (Nanjing, China). PVC sheet, sample pad, conjugate pad and absorption pad were purchased from Shanghai Kinbio Tech.Co.,ltd (Shanghai, China), Enrofloxacin, flumequine, danofloxacin, sparfloxacin, gatifloxacin, fleroxacin, lomefloxacin, difloxacin, sarafloxacin, ciprofloxacin, and norfloxacin were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). A commercial ENR ELISA test kit was purchased from Reagen LLC (Moorestown, NJ, USA). Anti-ENR polyclonal antibody (Ab) was produced in our laboratory.