Apc anti mouse cd11c antibody

To evaluate DC maturation in vitro, bone marrow cells were obtained from BALB/c mice and cultured in complete 1640 medium, supplemented with mouse recombinant macrophage colony stimulating factor (GMCSF, 20 ng/ml, Cat# 576304, Biolegend) and IL-4 (10 ng/ml, Cat# 574304, Biolegend) for 6 days to generate bone marrow-derived DCs. Then, IR-780 treated CT26 cancer cells were co-cultured with the DCs for 24 h. Next, the cells were collected and stained with APC anti-mouse CD11c Antibody (Cat# 117310, Biolegend), Brilliant Violet 421™ FITC anti-mouse CD80 Antibody (Cat# 104706, Biolegend), PE anti-mouse CD86 Antibody (Cat# 105008, Biolegend), or anti-mouse I-A/I-E Antibody (Cat# 107632, Biolegend), and analyzed by flow cytometry.

Anti-Rabbit SMYD2 (#9734), Anti-Mouse Lamin A/C (#4777), Anti-Rabbit RIG-I (#3743), Anti-Rabbit Phospho-IRF-3 (Ser396) (#4764), Anti-Rabbit TBK1 (#3013), Anti-Rabbit Phospho-TBK1/NAK (Ser172) (#5483), Anti-Rabbit MAVS (#4983), Anti-Rabbit NF-kappa-B p65 (#4764), Anti-Rabbit Phospho-NF-kappa-B p65 (Ser536) (#3033), Anti-Mouse Phospho-I-kappa-B-alpha (Ser32) (#2859), Anti-Rabbit IKK-alpha (#2682), Anti-Rabbit IKK-beta (#2684), Phospho-IKK-alpha (Ser176)/IKK-beta (Ser177) (#2078) and Anti-rabbit IgG-488 (#4412) were from Cell Signaling Technology. Anti-PP1-alpha (sc-7482) was from Santa Cruz. APC anti-mouse CD11c (117310) was from Biolegend. Anti-Rabbit PP2-alpha (610555), Percp-Rat Anti-Mouse CD4 (553052), FITC-Rat Anti-Mouse CD49b (553857) and PE Rat Anti-CD11b (553311) were from BD PharMingen. PE anti-mouse CD8a Antibody (100708), APC anti-mouse CD11c Antibody (117310), PerCP/Cy5.5 anti-mouse CD19 Antibody (115534), FITC anti-mouse Ly-6G (127606), FITC anti-mouse CD3 (100204), anti-mouse F/480 FITC (123107) and PE anti-mouse I-A/I-E (107608) were from Biolegend. Recombinant Mouse GM-CSF (415-ML-050) and Recombinant Mouse IL-4 Protein (404-ML-01M) were from R&D Systems. Murine M-CSF (315-02-1000) was from PeproTech. LYY507 was donated by the University of Oxford. Camptothecin (#13637) was from Cell Signaling Technology. ³H SAM (NET155H001MC) was from PerkinElmer.

A pharmaceutical-grade fucoidan extract from Marinova (batch number—DPFVF2015505), herein referred to as FE, with a purity of 98%, was purchased and used as received. Lipopolysaccharides (LPS from E. coli 055:B5) were purchased from InvivoGen Co., Ltd. (San Diego, CA, USA). Enzyme-linked immunosorbent assay (EISA) kits for murine IL-10 were purchased from NeoBioscience Co., Ltd. (Shenzhen, China). FITC anti-mouse F4/80 antibody and APC anti-mouse CD11C antibody for flow cytometric analysis were purchased from Biolegend (San Diego, CA, USA). Recombinant murine IFN-γ was purchased from Peprotech (Cranbury, NJ, USA). The mouse macrophage cell line Raw 264.7 was purchased from American-Type Culture Collection (ATCC, Manassas, VA, USA), and maintained according to the culture guidelines. All reagents used for the cell culture were purchased from Gibco Life Technology (Carlsbad, CA, USA).