Monoclonal anti nova1

Manufactured by Abnova

Monoclonal anti-Nova1 is a laboratory product used for the detection and analysis of the Nova1 protein. It is a purified monoclonal antibody that specifically binds to the Nova1 antigen. This product is intended for research use only and its core function is to provide a tool for the identification and study of the Nova1 protein in various experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

Monoclonal anti flag m2, by Merck Group (2 mentions) Monoclonal anti ptbp2, by Abnova (2 mentions) Ecl system, by Merck Group (2 mentions) Las 4000 imaging system, by Fujifilm (2 mentions) Monoclonal anti α tubulin, by Abcam (1 mentions) Monoclonal anti e cadherin, by Abcam (1 mentions) Polyclonal anti rbm4, by Santa Cruz Biotechnology (1 mentions) Polyclonal anti erk1 2, by Cell Signaling Technology (1 mentions)

2 protocols using monoclonal anti nova1

Immunoblot Analysis Protocol for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The immunoblot analysis was performed using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and results were monitored using the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). Primary antibodies used in this study included polyclonal anti-RBM416 (link), monoclonal anti-PTBP2 (Abnova, Taipei, Taiwan), monoclonal anti-SRSF6 (EMD, Millipore), monoclonal anti-Nova1 (Abnova), monoclonal anti-actin (Millipore), monoclonal anti-α-tubulin (Abcam, Cambridge, UK), monoclonal anti-E-cadherin (Abcam), monoclonal anti-N-cadherin (Abcam), and monoclonal anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA). Signal intensities were evaluated using TotalLab Quant Software (where available?).

Lin J.C., Lee Y.C., Liang Y.C., Fann Y.C., Johnson K.R, & Lin Y.J. (2017). The impact of the RBM4-initiated splicing cascade on modulating the carcinogenic signature of colorectal cancer cells. Scientific Reports, 7, 44204.

+ Open protocol

+ Expand

Quantitative Immunoblot Analysis Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols

The immunoblot analysis was conducted using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and images were analyzed with the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). Primary antibodies used in this study included polyclonal anti-RBM4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), monoclonal anti- PTBP2 (Abnova, Taipei, Taiwan), monoclonal anti-PTBP1 (EMD, Millipore), monoclonal anti-Nova1(Abnova), monoclonal anti-Actin (EMD, Millipore), monoclonal anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA), polyclonal anti-ERK1/2, and monoclonal anti-phospho-ERK1/2 (Cell Signaling Technology, Danvers, MA, USA). Intensities of the detected signals were determined using TotalLab Quant Software.

Lin J.C., Lu Y.H., Liu Y.R, & Lin Y.J. (2016). RBM4a-regulated splicing cascade modulates the differentiation and metabolic activities of brown adipocytes. Scientific Reports, 6, 20665.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!