For LC/MS analysis, 30 μL of patient serum was mixed with 470 μL of 85% methanol in water containing the internal standards (12.5 ng/mL kynurenine-d6, 100 ng/mL tryptophan-d5, and 12.5 ng/mL serotonin-d4). After removing proteins via centrifugation (18,440 or 21,210 ×g, 10 min, 4 °C), the solution was filtered with FastRemoverC18 (GL Science, Tokyo, Japan) using an automated system (Microlab NIMBUS with an MPE2 unit; Hamilton, Reno, NV, USA). The flow-through fraction was diluted 1:3 (v/v) with water and subjected to LC/MS analysis. The preparation method used for the serum samples of the healthy subjects was slightly different because of the low volumes of patient serum samples available for analysis. Proteins were removed using an automated system by mixing the serum (50 μL) with 450 μL of 90% methanol in water containing the internal standard and filtering with FastRemover for Protein (GL Science); the following procedure was the same as described above.
Fastremover for protein
Manufactured by GL Sciences
Sourced in Japan
FastRemover for Protein is a laboratory product designed to facilitate the removal of protein samples. It is a specialized tool intended for use in scientific and research environments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fastremover for protein
1
Serum Metabolite Profiling by LC/MS
2
Protein and Lipid Removal for IC-MS
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Extraction was performed using an automated system (Microlab NIMBUS, Hamilton Robotics, Reno, NV). The sample (20 µl) was mixed with 80 µl water and 400 µl methanol containing internal standard (2 µM hippuric acid-d5, Toronto Research Chemicals). Subsequently, 300 µl of the mixture was loaded onto the FastRemover for Protein (GL Sciences, Tokyo, Japan) to filter out protein coagulants. The flow-through solution (200 µl) was subsequently loaded onto the FastRemover C18 (GL Sciences) to remove lipids. Finally, 100 µl of flow-through solution was completely evaporated and stored at -80 °C until required for IC-MS analysis.
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