Polyamide sc 6

The NMR spectra were recorded on Bruker Avance DRX spectrometer at 400 MHz and 500 MHz (Bruker Scientific Instruments, MA, USA) using CDCl₃ and DMSO-d₆ (Sigma, St. Louis, Mo., USA). For Column chromatography (CC), several adsorbents were used, including Polyamide SC-6 (Macherey-Nagel, Düren, Germany), silica gel G₆₀ (60–120 mesh, Merck, Darmstadt, Germany), reversed phase RP-18 silica gel (E-Merck, Darmstadt, Germany), Sephadex LH-20 (25–100 mm mesh size, E-Merck, Darmstadt, Germany) and Diaion^® HP-20 (Sorbent Technologies, Norcross, GA, USA). Pre-coated silica G₆₀ F₂₅₄ 0.25 mm and RP-18 F₂₅₄ 0.25 mm (E-Merck, Darmstadt, Germany) were used for TLC. n-hexane, dichloromethane (CH₂Cl₂), ethyl acetate (EtOAc), and methanol (MeOH) were obtained from El-Nasr Pharmaceutical and Chemical Co., Egypt. MCF-7, HepG-2, and HCT-116 cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD). Dimethyl sulfoxide, vinblastine sulfate, MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and trypan blue dye were obtained from Sigma-Aldrich Chemical Co. (St. Louis, Mo., USA). Fetal bovine serum (Lonza, Belgium). RPMI-1640 (Roswell Park Memorial Institute) medium, DMEM and HEPES buffer solution, ʟ-Glutamine, Gentamycin, Trypsin-EDTA 0.25% were obtained from Lonza Bioscience (Belgium).