Siremadlin

FPFT-2216 and pomalidomide were synthesized by Fujimoto Pharmaceutical Group. Avadomide, iberdomide, siremadlin, idasanutlin, navtemadlin, milademetan, and MG-132 were procured from MedChemExpress; Z-VRPR-FMK and phorbol 12-myristate 13-acetate (PMA) were procured from Adipogen Life Sciences. Lenalidomide and ionomycin were obtained from Cayman Chemical, BAY 11-7082 from FUJIFILM Wako Pure Chemical Corporation, and safimaltib from Selleck Chemicals. Rituxan Intravenous Infusion (rituximab) was obtained from Zenyaku Kogyo. Z-VRPR-FMK was dissolved in PBS, and other reagents were dissolved in DMSO. The final DMSO concentration used for in vitro experiments did not exceed 0.25%.

Cell viability was detected by CCK8 assay (C6030, New Cell & Molecular Biotech Co., Ltd). Briefly, cells were seeded in 96-well plates with a density of 2 × 10³ cells/well. After treatments, CCK8 was added to the cell culture medium and maintained for additional 1 h. The absorbance was determined at 450 nm by a microplate reader (BioTek). Nutlin-3a (HY-10029), Siremadlin (HY-18658), Idasanutlin (HY-15676), and HY-100692 were purchased from MCE MedChemExpress. MI773 (S7649) was purchased from Selleck.

The human malignant rhabdoid tumor cell line JMU-RTK-2 was obtained from the JCRB cell bank and cultured in Dulbecco’s modified Eagle’s medium supplemented with 5% fetal bovine serum. The rhabdomyosarcoma cell line RMS-YM was obtained from RIKEN BRC cell bank and cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 20 mM-HEPES, and 0.1 mM None-essential amino acids (FUJIFILM Wako Pure Chemical Corporation). All the cells were maintained at 37 °C in a humidified atmosphere containing 5% CO₂. Topoisomerase inhibitors, etoposide, doxorubicin, and epirubicin were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), ellipticine was purchased from Merck (DS, Darmstadt, Germany), topotecan hydrochloride hydrate was purchased from Sigma Aldrich (St Louis, MO, USA), and siremadlin was purchased from Medchemexpress (Monmouth Junction, NJ, USA).