Staurosporine no s4400

HH-B2 (14 (link)), BCBL1 (7 (link)), and BC3 (60 (link)) cells were grown in RPMI 1640 medium (no. 11875085; Thermo Fisher Scientific) supplemented with 15% fetal bovine serum (FBS). HH-B2(Tet-On-F-ORF50) is a stable HH-B2 cell clone with a doxycycline-regulated ORF50 gene (61 (link)). For viral lytic induction, HH-B2 and BC3 cells were treated with 3 mM SB (no. B5887; Sigma-Aldrich), BCBL1 cells were treated with a combination of 3 mM SB and 30 ng/mL TPA (no. P8139; Sigma-Aldrich), and HH-B2(Tet-On-F-ORF50) cells were treated with 1 μg/mL doxycycline (no. D1822; Sigma-Aldrich). The T lymphocyte cell line Jurkat (62 (link)) was cultured in RPMI 1640 medium supplemented with 10% FBS. The human embryonic kidney cell line 293T (63 (link)) was cultured in Dulbecco’s modified Eagle medium (no. 11965084; Thermo Fisher Scientific) supplemented with 10% FBS. HKB5/B5 (17 (link)), a cell clone derived from 293T cells, was grown in RPMI 1640 medium supplemented with 8% FBS. Staurosporine (no. S4400), camptothecin (no. C9911), and dihydrotanshinone I (no. D0947) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Z-DEVD-FMK (no. FMK004) and Z-VAD (OMe)-FMK (no. tcsc3153) were purchased from R&D Systems (Indianapolis, IN, USA) and Taiclone (Taipei, Taiwan), respectively.

HH-B2 (14 (link)), BCBL1 (7 (link)), and BC3 (60 (link)) cells were grown in RPMI 1640 medium (no. 11875085; Thermo Fisher Scientific) supplemented with 15% fetal bovine serum (FBS). HH-B2(Tet-On-F-ORF50) is a stable HH-B2 cell clone with a doxycycline-regulated ORF50 gene (61 (link)). For viral lytic induction, HH-B2 and BC3 cells were treated with 3 mM SB (no. B5887; Sigma-Aldrich), BCBL1 cells were treated with a combination of 3 mM SB and 30 ng/mL TPA (no. P8139; Sigma-Aldrich), and HH-B2(Tet-On-F-ORF50) cells were treated with 1 μg/mL doxycycline (no. D1822; Sigma-Aldrich). The T lymphocyte cell line Jurkat (62 (link)) was cultured in RPMI 1640 medium supplemented with 10% FBS. The human embryonic kidney cell line 293T (63 (link)) was cultured in Dulbecco’s modified Eagle medium (no. 11965084; Thermo Fisher Scientific) supplemented with 10% FBS. HKB5/B5 (17 (link)), a cell clone derived from 293T cells, was grown in RPMI 1640 medium supplemented with 8% FBS. Staurosporine (no. S4400), camptothecin (no. C9911), and dihydrotanshinone I (no. D0947) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Z-DEVD-FMK (no. FMK004) and Z-VAD (OMe)-FMK (no. tcsc3153) were purchased from R&D Systems (Indianapolis, IN, USA) and Taiclone (Taipei, Taiwan), respectively.