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Sin3a

Manufactured by Cell Signaling Technology
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SIN3A is a protein that functions as a transcriptional regulator. It is a component of the SIN3 histone deacetylase complex, which is involved in the repression of gene expression.

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Lab products found in correlation

Hdac1, by Cell Signaling Technology (2 mentions) Hepg2, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) H3k4me2, by Active Motif (1 mentions) Penicillin streptomycin solution, by Merck Group (1 mentions) Jagged 1, by Cell Signaling Technology (1 mentions) Mono methyl arginine r gg, by Cell Signaling Technology (1 mentions) Pthrp, by Abcam (1 mentions) Prmt1, by Cell Signaling Technology (1 mentions) Runx1, by Abcam (1 mentions) Runx1, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Flag m2, by Merck Group (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Fgfr4, by Cell Signaling Technology (1 mentions) H3k36me3, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Fibroblast growth factor 2 (fgf2), by Cell Signaling Technology (1 mentions) H3k4me2, by Cell Signaling Technology (1 mentions) H3k9me2 3, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti‐SIN3A (2 citations)

3 protocols using sin3a

1

Transcriptional Regulation of Cell Lines

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HepG2, HuH7, T47D, and MCF-7 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and were not further tested or authenticated by the authors. The cell lines were maintained at 37°C in the presence of 5% CO2 in DMEM or RPMI medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution (Sigma Aldrich, St Louis). The cells (2.5 × 104 per well) were seeded, and the FBS content of the medium was reduced to 5%. Antibodies against β-actin, AHR, RNA Pol II and ER-α were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); NR2E3 was obtained from Aviva Systems Biology (San Diego, CA); Sin3A and LSD1 were purchased from Cell Signaling (Danvers, MA); H4Ac and H3K4me2 were procured from Active Motif, Carlsbad, CA). Benzo[a]pyrene (BaP) was dissolved in DMSO, and the stock solutions were directly added to the culture media. Control cells were treated with DMSO only.
Khnal T., Kim D., Johnson A., Choubey D, & Kim K. (2015). Deregulation of NR2E3, an orphan nuclear receptor, by benzo(a)pyrene-induced oxidative stress is associated with histone modification status change of the estrogen receptor gene promoter. Toxicology letters, 237(3), 228-236.
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2

Protein Analysis in Cell Signaling

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PAK4 (Cell Signaling Technology, USA); PAK4 (Santa cruz); Phospho-PAK4 (Ser474,Cell Signaling Technology, USA); RUNX1 (Abcam, USA), RUNX1 (Santa cruz), SIN3A (Cell Signaling Technology, USA); HDAC1 (Cell Signaling Technology, USA); PRMT1 (Cell Signaling Technology, USA); Mono-Methyl Arginine (R*GG) (Cell Signaling Technology, USA); Jagged-1 (Cell Signaling Technology, USA); PTHrP (Abcam, USA); Flag, GFP, GAPDH (GenScript, Nanjing, China).
Tang L., Gao Y., Song Y., Li Y., Li Y., Zhang H., Li D., Li J., Liu C, & Li F. (2020). PAK4 phosphorylating RUNX1 promotes ERα-positive breast cancer-induced osteolytic bone destruction. International Journal of Biological Sciences, 16(12), 2235-2247.
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3

Comprehensive Antibody and Growth Factor Panel

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The source of antibodies used in the study were as follows: AbCam H3K9ac (ab4441), H3K27Ac (ab4729). Cell Signaling Technologies AKT (9272), p-AKT (4056, 4058), CTBP1 (8684), ERK (9102), p-ERK (9101), FGFR4 (8562), GAPDH (2118), H3K4me3 (9751), H3K4me2 (9725), H3K36me3 (4909), H3K9me2/3 (5327), HA-tag (3724), HDAC1 (5356), HDAC2 (57156), Histone H3 (14269), JUN (9165), LSD1 (2139), MEK (4694), p-MEK (9154), pan-RAS (3965), RBBP7 (9067), RCOR1 (14567), SIN3A (8056), Stretavidin-HRP (3999). All CST antibodies were used at 1:1000 in 5% milk/PBST buffer. Santa Cruz c-MYC (sc-4084), HRAS (sc-34), KRAS (sc-30), NRAS (sc-519), TUBULIN (sc-69969). Santa Cruz antibodies were used at 1:500 except for TUBULIN at 1:3000. Sigma Aldrich FLAG-M2 (F1804, 1:1000), FGFR4 (HPA-028251, 1:500), RREB1 (HPA-001756, 1:500). The source of growth factors and mitogens were as follows: Cell Signaling Technologies EGF (8916), FGF1 (5234), FGF2 (61972), TGF-beta (8915), TNF-alpha (8902). Origene FGF8 (TP723101). Prospec HGF (cyt-244), FGF16 (cyt-939). Mek2 was a gift from Dustin Maly (Addgene plasmid # 40776; http://n2t.net/addgene:40776; RRID:Addgene_40776).
Kent O.A., Saha M., Coyaud E., Burston H.E., Law N., Dadson K., Chen S., Laurent E.M., St-Germain J., Sun R.X., Matsumoto Y., Cowen J., Montgomery-Song A., Brown K.R., Ishak C., Rose J.L., De Carvalho D.D., He H.H., Raught B., Billia F., Kannu P, & Rottapel R. (2020). Haploinsufficiency of RREB1 causes a Noonan-like RASopathy via epigenetic reprogramming of RAS-MAPK pathway genes. Nature Communications, 11, 4673.
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