Pepstatin A labels active cathepsin D inside lysosomes. For PepstatinA loading experiments, cells were loaded with 1 µM Pepstatin A-BODIPY® Conjugate (Life technologies, cat# P12271), for 30 min at 37 °C, and then fixed at 37 °C for 15 min via the addition of an equal volume of prewarmed, freshly made 8% PFA in 2× microtubule stabilization buffer (MTSB; 160 mM PIPES pH 6.8, 10 mM EGTA, 2 mM MgCl2) to minimize tubules rupture, as previously reported59 .
Alexa fluor 546 protein labeling kit
The Alexa Fluor™−546 Protein Labeling Kit is a laboratory tool designed for covalent labeling of proteins with the Alexa Fluor™ 546 fluorescent dye. The kit provides the necessary components to facilitate efficient labeling of proteins for various analytical and experimental purposes.
Lab products found in correlation
4 protocols using alexa fluor 546 protein labeling kit
Alexa Fluor Labeling and Lysosomal Trafficking Assay
Pepstatin A labels active cathepsin D inside lysosomes. For PepstatinA loading experiments, cells were loaded with 1 µM Pepstatin A-BODIPY® Conjugate (Life technologies, cat# P12271), for 30 min at 37 °C, and then fixed at 37 °C for 15 min via the addition of an equal volume of prewarmed, freshly made 8% PFA in 2× microtubule stabilization buffer (MTSB; 160 mM PIPES pH 6.8, 10 mM EGTA, 2 mM MgCl2) to minimize tubules rupture, as previously reported59 .
Fluorescent Tumor Imaging Cocktail
Cytoskeletal Regulators in Cell Dynamics
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