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Gel 60 tlc aluminum plate

Manufactured by Merck Group
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Sourced in Germany

The Gel-60 TLC aluminum plate is a thin-layer chromatography (TLC) plate made of aluminum substrate with a silica gel 60 adsorbent layer. It is designed for qualitative and semi-quantitative analysis of various compounds through chromatographic separation.

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Lab products found in correlation

Quantity one, by Bio-Rad (1 mentions)

Close spelling variants of this product

TLC silica gel 60 F 254 aluminum plates Aluminum silica gel 60 F254 TLC plates Aluminum TLC plates TLC plates

2 protocols using gel 60 tlc aluminum plate

1

Carbon Source Degradation Analysis

Cited 1 time
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The carbon source degradation products were detected by thin-layer chromatography (TLC) analysis. Briefly, samples (0.2 mL) were loaded onto a pre-coated silica gel-60 TLC aluminum plate (Merck, Darmstadt, Germany). After being developed with a solvent system consisting of formic acid/n-butanol/water (6:4:1, v:v:v), the plate was soaked in orcinol reagent (Sigma-Aldrich Co., LLC., St. Louis, MO, USA) and visualized at 120 °C for 1 min [8 (link)]. The scanned TLC profiles were analyzed by Quantity One (BioRad, Hercules, CA, USA) and the amounts of carbon source in each sample were quantified. Degradation rates were calculated as the percentage of total amounts at 0 h subtracted from those at 24 h after fermentation.
Li Y., Jiang J., Zhu L., Wang X., Wan W., Wang D, & Li Z. (2022). Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System. Microorganisms, 10(7), 1453.
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2

TLC Analysis of Carbohydrate Degradation

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To perform TLC, 0.2 μL of each sample was loaded on a pre-coated silica gel-60 TLC aluminum plate (Merck, Germany). After development with a solvent system consisting of formic acid, n-butanol, and water (6:4:1, v:v:v), the plate was soaked in orcinol reagent, and carbohydrates were visualized by heating at 120 C for 3 min.
To calculate the degradation rates of these carbohydrates, IntDen of each dot at the spot site was measured using ImageJ software (https://imagej.nih.gov/ij; National Institutes of Health, Bethesda, MD, USA). Then the degradation rates were calculated by comparing to control (0 h).
Yin Y., Li M., Gu W., Zeng B., Liu W., Zhu L., Pi X., Primerano D.A., Yu H.D., Wei H., Yu G, & Wang X. (2021). Carrageenan oligosaccharides and associated carrageenan-degrading bacteria induce intestinal inflammation in germ-free mice. Journal of genetics and genomics = Yi chuan xue bao, 48(9), 815-824.
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