Dulbecco s modified eagle s medium nutrient mixture f 12 dmem f 12 glutamax

Milli-Q water was obtained from a Millipore unit. EtOH non denaturalized (99.9%) was bought from Dávila Villalobos S.L. (Valladolid, Spain), N₂ (99.996%) from Linde Gas (Puçol, Spain), Fluorescein sodium salt from Vetec Química (Xerem Duque De Caxias, Rio de Janeiro, Brazil) and OxiSelect 96-Well Comet Assay Kit from bioNova scientific (Fremont, CA, USA). Methanol (MeOH, 99.9% LC-MS), n-hexane (95%), NaOH pellets, Dimethyl Sulfoxide (DMSO), and phosphoric acid were supplied by Panreac Quimica SLU (Barcelona, Spain), while commercial standards HT (≥98%), TY (≥99%) and OL (≥98%) by Extrasynthese (Genay, France). MgSO₄ anhydrous, KI, NaBr, NaCl, Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), AAPH (2,2′-azobis(2-methylpropionamidine)dihydrochloride), gallic acid, catechin, Tris-EDTA buffer solution, and bovine insulin were purchased from Sigma-Aldrich (Madrid, Spain). Plastic culture flasks, plates, tips, and pipettes, Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F-12) + GlutaMax, Dulbecco’s phosphate-buffered saline, fetal bovine serum, human epithelial growth factor, human insulin, penicillin, and streptomycin were supplied by Thermo Fisher Scientific (Rockford, IL, USA). CO₂ (99.95%) was obtained by Carburos Metálicos (Barcelona, Spain).

For the preparation of the phenolic extracts, MilliQ water was obtained from a Millipore unit, ethanol (EtOH) non denaturalized (99.9%) from Dávila Villalobos S.L. (Valladolid, Spain), CO₂ (99.95%) from Carburos Metálicos (Barcelona, Spain), and N₂ (99.996%) from Linde Gas (Dublin, Ireland). HT (≥98%) and OL (≥98%) were supplied by Extrasynthese (Genay, France). For the cell-based assays, plastic culture flasks, plates, tips and pipettes, Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F-12) + GlutaMax, DMEM/F12 without phenol red, Dulbecco’s phosphate buffered saline (DPBS), fetal bovine serum (FBS), human epithelial growth factor (EGF), human insulin, penicillin, streptomycin, and bicinchoninic acid (BCA) assay kit were purchased from Thermo Fisher Scientific (Rockford, IL, USA); cytokine TNF-α from bioNova scientific (Fremont, CA, US); bovine insulin, 20,70-dichlorodihydrofluorescein diacetate (H2DCF-DA), 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) and 5-methylphenazinium methyl sulfate (PMS) reagents, dimethyl sulfoxide (DMSO), and Milliplex Human Cytokine/Chemokine HCYTOMAG-60K-5 plex Magnetic Kit (IL-1beta, IL-6, IL-8/CXCL8, IL-17A, and IP-10) from Merck Life Science (St. Louis, MO, USA).